How to predict structures with AlphaFold: Difference between revisions
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</ref>. In 2024, ColabFold is not necessarily the best or only place to submit you job: see [[#Prediction Servers]]. | </ref>. In 2024, ColabFold is not necessarily the best or only place to submit you job: see [[#Prediction Servers]]. | ||
Initially, AlphaFold and ColabFold performed best with '''single chains'''<ref name=" | Initially, AlphaFold and ColabFold performed best with '''single chains'''<ref name="afmultimer">[https://www.biorxiv.org/content/10.1101/2021.10.04.463034v1 Protein complex prediction with AlphaFold-Multimer], 2021, Evans ''et al.'' (DeepMind Team).</ref>, which may include one or a few domains. The instructions below were written '''before ColabFold was adapted to prediction of multimers'''. If you are interested in complexes or alternate conformations, please see ColabFold instructions in the 2023 paper by Kim ''et al.'' <ref name="kim2023">[https://protocolexchange.researchsquare.com/article/pex-2490/v1 Easy and accurate protein structure prediction using ColabFold], 2023, Kim ''et al.'' (DeepMind Team).</ref> | ||
===Submitting A Sequence=== | ===Submitting A Sequence=== | ||