1xt9: Difference between revisions

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[[Image:1xt9.gif|left|200px]]<br />

[[Image:1xt9.gif|left|200px]]<br /><applet load="1xt9" size="350" color="white" frame="true" align="right" spinBox="true"

<applet load="1xt9" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

caption="1xt9, resolution 2.20Å" />

'''Crystal Structure of Den1 in complex with Nedd8'''<br />

==Overview==

The Nedd8 conjugation pathway is conserved from yeast to humans and is, essential in many organisms. Nedd8 is conjugated to cullin proteins in a, process that alters SCF E3 ubiquitin ligase activity, and it is presumed, that Nedd8 deconjugation would reverse these effects. We now report the, X-ray structures of the human Nedd8-specific protease, Den1, in a complex, with the inhibitor Nedd8 aldehyde, thus revealing a model for the, tetrahedral transition state intermediate generated during proteolysis., Although Den1 is closely related to the SUMO-specific protease family, (Ulp/Senp family), structural analysis of the interface suggests, determinants involved in Nedd8 selectivity by Den1 over other, ubiquitin-like family members and suggests how the Ulp/Senp architecture, has been modified to interact with different ubiquitin-like modifiers.

The Nedd8 conjugation pathway is conserved from yeast to humans and is essential in many organisms. Nedd8 is conjugated to cullin proteins in a process that alters SCF E3 ubiquitin ligase activity, and it is presumed that Nedd8 deconjugation would reverse these effects. We now report the X-ray structures of the human Nedd8-specific protease, Den1, in a complex with the inhibitor Nedd8 aldehyde, thus revealing a model for the tetrahedral transition state intermediate generated during proteolysis. Although Den1 is closely related to the SUMO-specific protease family (Ulp/Senp family), structural analysis of the interface suggests determinants involved in Nedd8 selectivity by Den1 over other ubiquitin-like family members and suggests how the Ulp/Senp architecture has been modified to interact with different ubiquitin-like modifiers.

==About this Structure==

1XT9 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1XT9 OCA].

1XT9 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XT9 OCA].

==Reference==

Line 14:

Line 13:

[[Category: Homo sapiens]]

[[Category: Protein complex]]

[[Category: Erdene, T.G.]]

[[Category: Erdene, T G.]]

[[Category: Lima, C.D.]]

[[Category: Lima, C D.]]

[[Category: Pan, Z.Q.]]

[[Category: Pan, Z Q.]]

[[Category: Reverter, D.]]

[[Category: Wilkinson, K.D.]]

[[Category: Wilkinson, K D.]]

[[Category: Wu, K.]]

[[Category: cysteine protease]]

[[Category: ubiquitin-like]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Mon Nov 12 20~~:10:~~33 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:58:25 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1xt9.gif|left|200px]]<br />
+[[Image:1xt9.gif|left|200px]]<br /><applet load="1xt9" size="350" color="white" frame="true" align="right" spinBox="true"
-<applet load="1xt9" size="450" color="white" frame="true" align="right" spinBox="true"
 caption="1xt9, resolution 2.20&Aring;" />
 '''Crystal Structure of Den1 in complex with Nedd8'''<br />
 ==Overview==
-The Nedd8 conjugation pathway is conserved from yeast to humans and is, essential in many organisms. Nedd8 is conjugated to cullin proteins in a, process that alters SCF E3 ubiquitin ligase activity, and it is presumed, that Nedd8 deconjugation would reverse these effects. We now report the, X-ray structures of the human Nedd8-specific protease, Den1, in a complex, with the inhibitor Nedd8 aldehyde, thus revealing a model for the, tetrahedral transition state intermediate generated during proteolysis., Although Den1 is closely related to the SUMO-specific protease family, (Ulp/Senp family), structural analysis of the interface suggests, determinants involved in Nedd8 selectivity by Den1 over other, ubiquitin-like family members and suggests how the Ulp/Senp architecture, has been modified to interact with different ubiquitin-like modifiers.
+The Nedd8 conjugation pathway is conserved from yeast to humans and is essential in many organisms. Nedd8 is conjugated to cullin proteins in a process that alters SCF E3 ubiquitin ligase activity, and it is presumed that Nedd8 deconjugation would reverse these effects. We now report the X-ray structures of the human Nedd8-specific protease, Den1, in a complex with the inhibitor Nedd8 aldehyde, thus revealing a model for the tetrahedral transition state intermediate generated during proteolysis. Although Den1 is closely related to the SUMO-specific protease family (Ulp/Senp family), structural analysis of the interface suggests determinants involved in Nedd8 selectivity by Den1 over other ubiquitin-like family members and suggests how the Ulp/Senp architecture has been modified to interact with different ubiquitin-like modifiers.
 ==About this Structure==
-XT9 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1XT9 OCA].
+XT9 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XT9 OCA].
 ==Reference==
@@ Line 14: / Line 13: @@
 [[Category: Homo sapiens]]
 [[Category: Protein complex]]
-[[Category: Erdene, T.G.]]
+[[Category: Erdene, T G.]]
-[[Category: Lima, C.D.]]
+[[Category: Lima, C D.]]
-[[Category: Pan, Z.Q.]]
+[[Category: Pan, Z Q.]]
 [[Category: Reverter, D.]]
-[[Category: Wilkinson, K.D.]]
+[[Category: Wilkinson, K D.]]
 [[Category: Wu, K.]]
 [[Category: cysteine protease]]
 [[Category: ubiquitin-like]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 20:10:33 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:58:25 2008''