9k09: Difference between revisions

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'''Unreleased structure'''


The entry 9k09 is ON HOLD  until Paper Publication
==Cyanophage A4 portal-tail complex==
<StructureSection load='9k09' size='340' side='right'caption='[[9k09]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9k09]] is a 48 chain structure with sequence from [https://en.wikipedia.org/wiki/Anabaena_phage_A-4L Anabaena phage A-4L]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9K09 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9K09 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9k09 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9k09 OCA], [https://pdbe.org/9k09 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9k09 RCSB], [https://www.ebi.ac.uk/pdbsum/9k09 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9k09 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A059PY41_9CAUD A0A059PY41_9CAUD]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Upon infection, the podophages usually eject a couple of proteins from the capsid to form a transmembrane ejectosome on the host cell membrane that facilitates the ejection of viral genome. However, it remains unclear how these proteins of pre-ejectosome are finely assembled at the center of highly packaged genome. Here, we report the intact structure of Anabaena cyanopodophage A4, which consists of a capsid stabilized by two types of cement proteins and a short tail attached with six tail fibers. Notably, we find a pentameric pre-ejectosome at the core of capsid, which is composed of four ejection proteins wrapped into a coaxial cylinder of triple layers. Moreover, a segment of genomic DNA runs along the positively charged circular cleft formed by two ejection proteins. Based on the mortise-and-tenon architecture of pre-ejectosome in combination with previous studies, we propose a putative DNA packaging process and ejection mechanism for podophages. These findings largely enrich our knowledge on the assembly mechanism of podophages, which might facilitate the application of A4 as a chassis cyanophage in synthetic biology.


Authors:  
Cryo-EM structure of cyanopodophage A4 reveals a pentameric pre-ejectosome in the double-stabilized capsid.,Hou P, Zhou RQ, Jiang YL, Yu RC, Du K, Gan N, Ke F, Zhang QY, Li Q, Zhou CZ Proc Natl Acad Sci U S A. 2025 Apr 8;122(14):e2423403122. doi: , 10.1073/pnas.2423403122. Epub 2025 Mar 31. PMID:40163721<ref>PMID:40163721</ref>


Description:  
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
<div class="pdbe-citations 9k09" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Anabaena phage A-4L]]
[[Category: Large Structures]]
[[Category: Hou P]]
[[Category: Li Q]]
[[Category: Zhou CZ]]
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