1ubi: Difference between revisions

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[[Image:1ubi.~~jpg~~|left|200px]]

[[Image:1ubi.png|left|200px]]

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~~'''~~SYNTHETIC STRUCTURAL AND BIOLOGICAL STUDIES OF THE UBIQUITIN SYSTEM. PART 1~~'''~~

===SYNTHETIC STRUCTURAL AND BIOLOGICAL STUDIES OF THE UBIQUITIN SYSTEM. PART 1===

~~==Overview==~~

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~~The small protein ubiquitin (76 amino acids) has been synthesized under optimized conditions by Merrifield solid~~-~~phase methodology using the N alpha~~-~~Fmoc protecting group.~~ The ~~crude polypeptide mixture was purified to homogeneity by gel filtration~~, ~~dialysis and a combination of cation- and anion-exchange chromatography to yield ubiquitin. Amino acid analysis~~, ~~enzymic digestion and sequencing by automated Edman degradation were used to authenticate~~ the ~~primary structure. Isoelectric focusing and m.s. were used~~ to ~~demonstrate that~~ the ~~final product was greater than 98% pure with a final yield of 93 mg~~ (4.3%) ~~from a single synthesis on a 0.25 nmol scale~~.

The line below this paragraph, {{ABSTRACT_PUBMED_8166633}}, adds the Publication Abstract to the page

(as it appears on PubMed at http://www.pubmed.gov), where 8166633 is the PubMed ID number.

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==About this Structure==

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[[Category: Turner, M A.]]

[[Category: Chromosomal protein]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sat May 3 11~~:00:09 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 09:25:19 2008''

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-[[Image:1ubi.jpg|left|200px]]
+{{Seed}}
+[[Image:1ubi.png|left|200px]]
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 {{STRUCTURE_1ubi|  PDB=1ubi  |  SCENE=  }}
-'''SYNTHETIC STRUCTURAL AND BIOLOGICAL STUDIES OF THE UBIQUITIN SYSTEM. PART 1'''
+===SYNTHETIC STRUCTURAL AND BIOLOGICAL STUDIES OF THE UBIQUITIN SYSTEM. PART 1===
-==Overview==
+<!--
-The small protein ubiquitin (76 amino acids) has been synthesized under optimized conditions by Merrifield solid-phase methodology using the N alpha-Fmoc protecting group. The crude polypeptide mixture was purified to homogeneity by gel filtration, dialysis and a combination of cation- and anion-exchange chromatography to yield ubiquitin. Amino acid analysis, enzymic digestion and sequencing by automated Edman degradation were used to authenticate the primary structure. Isoelectric focusing and m.s. were used to demonstrate that the final product was greater than 98% pure with a final yield of 93 mg (4.3%) from a single synthesis on a 0.25 nmol scale.
+The line below this paragraph, {{ABSTRACT_PUBMED_8166633}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 8166633 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_8166633}}
 ==About this Structure==
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 [[Category: Turner, M A.]]
 [[Category: Chromosomal protein]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 11:00:09 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 09:25:19 2008''