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| {{STRUCTURE_1ug4| PDB=1ug4 | SCENE= }} | | {{STRUCTURE_1ug4| PDB=1ug4 | SCENE= }} |
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| '''Crystal Structure of Cardiotoxin VI from Taiwan Cobra (Naja atra) Venom'''
| | ===Crystal Structure of Cardiotoxin VI from Taiwan Cobra (Naja atra) Venom=== |
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| ==Overview==
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| Natural homologues of cobra cardiotoxins (CTXs) were classified into two structural subclasses of group I and II based on the amino acid sequence and circular dichroism analysis, but the exact differences in their three-dimensional structures and biological significance remain elusive. We show by circular dichroism, NMR spectroscopic, and X-ray crystallographic analyses of a newly purified group I CTX A6 from eastern Taiwan cobra (Naja atra) venoms that its loop I conformation adopts a type VIa turn with a cis peptide bond located between two proline residues of PPxY. A similar "banana-twisted" conformation can be observed in other group I CTXs and also in cyclolinopeptide A and its analogues. By binding to the membrane environment, group I CTX undergoes a conformational change to adopt a more extended hydrophobic domain with beta-sheet twisting closer to the one adopted by group II CTX. This result resolves a discrepancy in the CTX structural difference reported previously between solution as well as crystal state and shows that, in addition to the hydrophobicity, the exact loop I conformation also plays an important role in CTX-membrane interaction. Potential protein targets of group I CTXs after cell internalization are also discussed on the basis of the determined loop I conformation.
| | The line below this paragraph, {{ABSTRACT_PUBMED_15895985}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 15895985 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_15895985}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Cobra]] | | [[Category: Cobra]] |
| [[Category: Venom]] | | [[Category: Venom]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 11:10:51 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 23:46:52 2008'' |
