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| {{STRUCTURE_1ukc| PDB=1ukc | SCENE= }} | | {{STRUCTURE_1ukc| PDB=1ukc | SCENE= }} |
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| '''Crystal Structure of Aspergillus niger EstA'''
| | ===Crystal Structure of Aspergillus niger EstA=== |
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| ==Overview==
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| From the fungus Aspergillus niger, we identified a new gene encoding protein EstA, a member of the alpha/beta-hydrolase fold superfamily but of unknown substrate specificity. EstA was overexpressed and its crystal structure was solved by molecular replacement using a lipase-acetylcholinesterase chimera template. The 2.1 A resolution structure of EstA reveals a canonical Ser/Glu/His catalytic triad located in a small pocket at the bottom of a large solvent-accessible, bowl-shaped cavity. Potential substrates selected by manual docking procedures were assayed for EstA activity. Consistent with the pocket geometry, preference for hydrolysis of short acyl/propyl chain substrates was found. Identification of close homologs from the genome of other fungi, of which some are broad host-range pathogens, defines EstA as the first member of a novel class of fungal esterases within the superfamily. Hence the structure of EstA constitutes a lead template in the design of new antifungal agents directed toward its pathogenic homologs.
| | The line below this paragraph, {{ABSTRACT_PUBMED_15062090}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 15062090 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_15062090}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Esterase]] | | [[Category: Esterase]] |
| [[Category: Fungi]] | | [[Category: Fungi]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 11:20:59 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 20:18:39 2008'' |
