9hnw: Difference between revisions

Latest revision as of 06:28, 12 February 2025

USP1-UAF1 bound to Lys63-linked diubiquitin

Structural highlights

9hnw is a 4 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Electron Microscopy, Resolution 3.04Å
Ligands:	ABU, DAB, ZN
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

UBP1_HUMAN Negative regulator of DNA damage repair which specifically deubiquitinates monoubiquitinated FANCD2 (PubMed:15694335). Also involved in PCNA-mediated translesion synthesis (TLS) by deubiquitinating monoubiquitinated PCNA (PubMed:16531995, PubMed:20147293). Has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have a high activity (PubMed:18082604, PubMed:26388029).^[1] ^[2] ^[3] ^[4] ^[5]

References

↑ Nijman SM, Huang TT, Dirac AM, Brummelkamp TR, Kerkhoven RM, D'Andrea AD, Bernards R. The deubiquitinating enzyme USP1 regulates the Fanconi anemia pathway. Mol Cell. 2005 Feb 4;17(3):331-9. PMID:15694335 doi:10.1016/j.molcel.2005.01.008
↑ Huang TT, Nijman SM, Mirchandani KD, Galardy PJ, Cohn MA, Haas W, Gygi SP, Ploegh HL, Bernards R, D'Andrea AD. Regulation of monoubiquitinated PCNA by DUB autocleavage. Nat Cell Biol. 2006 Apr;8(4):339-47. PMID:16531995 doi:10.1038/ncb1378
↑ Cohn MA, Kowal P, Yang K, Haas W, Huang TT, Gygi SP, D'Andrea AD. A UAF1-containing multisubunit protein complex regulates the Fanconi anemia pathway. Mol Cell. 2007 Dec 14;28(5):786-97. PMID:18082604 doi:https://dx.doi.org/10.1016/j.molcel.2007.09.031
↑ Lee KY, Yang K, Cohn MA, Sikdar N, D'Andrea AD, Myung K. Human ELG1 regulates the level of ubiquitinated proliferating cell nuclear antigen (PCNA) through Its interactions with PCNA and USP1. J Biol Chem. 2010 Apr 2;285(14):10362-9. PMID:20147293 doi:10.1074/jbc.M109.092544
↑ Yin J, Schoeffler AJ, Wickliffe K, Newton K, Starovasnik MA, Dueber EC, Harris SF. Structural Insights into WD-Repeat 48 Activation of Ubiquitin-Specific Protease 46. Structure. 2015 Sep 12. pii: S0969-2126(15)00359-7. doi:, 10.1016/j.str.2015.08.010. PMID:26388029 doi:https://dx.doi.org/10.1016/j.str.2015.08.010

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Nijman SM, Huang TT, Dirac AM, Brummelkamp TR, Kerkhoven RM, D'Andrea AD, Bernards R. The deubiquitinating enzyme USP1 regulates the Fanconi anemia pathway. Mol Cell. 2005 Feb 4;17(3):331-9. PMID:15694335 doi:10.1016/j.molcel.2005.01.008

[2] Huang TT, Nijman SM, Mirchandani KD, Galardy PJ, Cohn MA, Haas W, Gygi SP, Ploegh HL, Bernards R, D'Andrea AD. Regulation of monoubiquitinated PCNA by DUB autocleavage. Nat Cell Biol. 2006 Apr;8(4):339-47. PMID:16531995 doi:10.1038/ncb1378

[3] Cohn MA, Kowal P, Yang K, Haas W, Huang TT, Gygi SP, D'Andrea AD. A UAF1-containing multisubunit protein complex regulates the Fanconi anemia pathway. Mol Cell. 2007 Dec 14;28(5):786-97. PMID:18082604 doi:https://dx.doi.org/10.1016/j.molcel.2007.09.031

[4] Lee KY, Yang K, Cohn MA, Sikdar N, D'Andrea AD, Myung K. Human ELG1 regulates the level of ubiquitinated proliferating cell nuclear antigen (PCNA) through Its interactions with PCNA and USP1. J Biol Chem. 2010 Apr 2;285(14):10362-9. PMID:20147293 doi:10.1074/jbc.M109.092544

[5] Yin J, Schoeffler AJ, Wickliffe K, Newton K, Starovasnik MA, Dueber EC, Harris SF. Structural Insights into WD-Repeat 48 Activation of Ubiquitin-Specific Protease 46. Structure. 2015 Sep 12. pii: S0969-2126(15)00359-7. doi:, 10.1016/j.str.2015.08.010. PMID:26388029 doi:https://dx.doi.org/10.1016/j.str.2015.08.010

[1]

[2]

[3]

[4]

[5]

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 9hnw is ON HOLD
+==USP1-UAF1 bound to Lys63-linked diubiquitin==
+<StructureSection load='9hnw' size='340' side='right'caption='[[9hnw]], [[Resolution|resolution]] 3.04&Aring;' scene=''>
-Authors: Keijzer, N., Sakoltchik, J., Sixma, T.K.
+== Structural highlights ==
+<table><tr><td colspan='2'>[[9hnw]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9HNW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9HNW FirstGlance]. <br>
-Description: USP1-UAF1 bound to Lys63-linked diubiquitin
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.04&#8491;</td></tr>
-[[Category: Unreleased Structures]]
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ABU:GAMMA-AMINO-BUTANOIC+ACID'>ABU</scene>, <scene name='pdbligand=DAB:2,4-DIAMINOBUTYRIC+ACID'>DAB</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-[[Category: Keijzer, N]]
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9hnw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9hnw OCA], [https://pdbe.org/9hnw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9hnw RCSB], [https://www.ebi.ac.uk/pdbsum/9hnw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9hnw ProSAT]</span></td></tr>
-[[Category: Sixma, T.K]]
+</table>
-[[Category: Sakoltchik, J]]
+== Function ==
+[https://www.uniprot.org/uniprot/UBP1_HUMAN UBP1_HUMAN] Negative regulator of DNA damage repair which specifically deubiquitinates monoubiquitinated FANCD2 (PubMed:15694335). Also involved in PCNA-mediated translesion synthesis (TLS) by deubiquitinating monoubiquitinated PCNA (PubMed:16531995, PubMed:20147293). Has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have a high activity (PubMed:18082604, PubMed:26388029).<ref>PMID:15694335</ref> <ref>PMID:16531995</ref> <ref>PMID:18082604</ref> <ref>PMID:20147293</ref> <ref>PMID:26388029</ref>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Homo sapiens]]
+[[Category: Large Structures]]
+[[Category: Keijzer N]]
+[[Category: Sakoltchik J]]
+[[Category: Sixma TK]]

9hnw: Difference between revisions

Latest revision as of 06:28, 12 February 2025

USP1-UAF1 bound to Lys63-linked diubiquitin

Structural highlights

Function

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools