9kov: Difference between revisions

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'''Unreleased structure'''


The entry 9kov is ON HOLD  until Paper Publication
==Crystal structure of an amidohydrolase mutant from Thermonema==
<StructureSection load='9kov' size='340' side='right'caption='[[9kov]], [[Resolution|resolution]] 2.56&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9kov]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrinomonas_methylaliphatogenes Pyrinomonas methylaliphatogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9KOV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9KOV FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.56&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9kov FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9kov OCA], [https://pdbe.org/9kov PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9kov RCSB], [https://www.ebi.ac.uk/pdbsum/9kov PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9kov ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Ochratoxin A (OTA) is highly toxic and widely distributed, posing serious threats to human and animal health. Searching for effective OTA-detoxifying enzyme is crucial for the prevention and control of OTA contaminations. Here, a new OTA-detoxifying enzyme, TrADH from Thermonema rossianum is identified, which exhibits highest temperature tolerance among OTA-detoxifying enzymes. TrADH maintains good activity in the range of 45-85 degrees C and retains about 50 % activity after heating at 70 degrees C for 30 min. Based on the solved crystal structures, the catalytic mechanism is proposed, and protein engineering of catalytic-related residues is performed to obtain a 2.1-fold upgraded variant TrADHS67E with the specific enzyme activity of 3990 U/mg, which is more efficient than the reported OTA-detoxifying enzymes. The efficient degradation of OTA in rum and walnut reveals the prospect of TrADH in food applications. The results indicate that TrADH has the potential in OTA bio-detoxification in food and feed industry.


Authors: Xu, N.N., Jian, G., Wei, H.L., Chen, Y.Y., Wu, P., Han, X., Liu, W.
A thermostable OTA-detoxifying hydrolase from Thermonema rossianum: identification, characterization, structure, catalytic mechanism, and application.,Xu N, Yan M, Liang X, Qin H, Gao J, Liu W Food Chem. 2025 Sep 1;485:144515. doi: 10.1016/j.foodchem.2025.144515. Epub 2025 , Apr 25. PMID:40318335<ref>PMID:40318335</ref>


Description: Crystal structure of an amidohydrolase mutant from Thermonema
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
[[Category: Wei, H.L]]
<div class="pdbe-citations 9kov" style="background-color:#fffaf0;"></div>
[[Category: Chen, Y.Y]]
== References ==
[[Category: Liu, W]]
<references/>
[[Category: Xu, N.N]]
__TOC__
[[Category: Han, X]]
</StructureSection>
[[Category: Wu, P]]
[[Category: Large Structures]]
[[Category: Jian, G]]
[[Category: Pyrinomonas methylaliphatogenes]]
[[Category: Chen YY]]
[[Category: Han X]]
[[Category: Jian G]]
[[Category: Liu W]]
[[Category: Wei HL]]
[[Category: Wu P]]
[[Category: Xu NN]]
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