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'''Unreleased structure'''


The entry 9l36 is ON HOLD
==Cryo-electron microscopic structure of a novel amidohydrolase ADH3 triple mutation==
<StructureSection load='9l36' size='340' side='right'caption='[[9l36]], [[Resolution|resolution]] 2.71&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9l36]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Stenotrophomonas_sp._CW117 Stenotrophomonas sp. CW117]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9L36 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9L36 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.71&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=97U:(2~{S})-2-[[(3~{R})-5-chloranyl-3-methyl-8-oxidanyl-1-oxidanylidene-3,4-dihydroisochromen-7-yl]carbonylamino]-3-phenyl-propanoic+acid'>97U</scene>, <scene name='pdbligand=KCX:LYSINE+NZ-CARBOXYLIC+ACID'>KCX</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9l36 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9l36 OCA], [https://pdbe.org/9l36 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9l36 RCSB], [https://www.ebi.ac.uk/pdbsum/9l36 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9l36 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A0S1B1B6_9GAMM A0A0S1B1B6_9GAMM]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Ochratoxin A (OTA) is a pervasive and significant mycotoxin that poses serious health risks to humans and animals. The development of efficient biocatalysts for the enzymatic detoxification of OTA is of great importance. In this study, we enhanced the OTA-degrading activity of three amidohydrolases (ADHs) by up to ninefold. This improvement was achieved by reducing steric hindrance in the binding pocket and fine-tuning the hydrophilic interactions between the enzyme and substrate. The most efficient variant, PwADH/DM, was immobilized onto magnetic Fe(3)O(4) nanoparticles functionalized by the co-deposition of dopamine and polyethyleneimine. Under optimal conditions, this immobilization process achieved a high immobilization efficiency (85.4%) and activity recovery (76.9%). The immobilized enzyme exhibited enhanced pH stability and thermostability, along with good storage stability, reusability, and recyclability. More importantly, the immobilized enzyme completely degraded 100 ng/mL OTA in contaminated milk without affecting the milk's properties. These findings expand our understanding of the molecular mechanisms governing substrate binding and catalysis in OTA-degrading ADHs. Furthermore, they provide a blueprint for enzyme-based OTA decontamination during food processing.


Authors: Dai, L.H., He, B.Y., Hu, Y.M., Xu, Y.H., Huang, J.P., Xie, Z.Z., Li, H., Niu, D., Guo, R.-T., Chen, C.-C.
Structure-guided protein engineering and immobilization of an amidohydrolase for efficient ochratoxin A detoxification.,Hu Y, Xu Y, He B, Niu D, Yang X, Huang J, Xie Z, Shen P, Li X, Bai M, Liu Z, Li H, Yang Y, Huang JW, Chen CC, Guo RT, Dai L Int J Biol Macromol. 2026 Apr;358:151671. doi: 10.1016/j.ijbiomac.2026.151671. , Epub 2026 Mar 26. PMID:41903634<ref>PMID:41903634</ref>


Description: Cryo-electron microscopic structure of a novel amidohydrolase ADH3 triple mutation
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
[[Category: Huang, J.P]]
<div class="pdbe-citations 9l36" style="background-color:#fffaf0;"></div>
[[Category: Chen, C.-C]]
== References ==
[[Category: He, B.Y]]
<references/>
[[Category: Xie, Z.Z]]
__TOC__
[[Category: Hu, Y.M]]
</StructureSection>
[[Category: Dai, L.H]]
[[Category: Large Structures]]
[[Category: Xu, Y.H]]
[[Category: Stenotrophomonas sp. CW117]]
[[Category: Li, H]]
[[Category: Chen C-C]]
[[Category: Guo, R.-T]]
[[Category: Dai LH]]
[[Category: Niu, D]]
[[Category: Guo R-T]]
[[Category: He BY]]
[[Category: Hu YM]]
[[Category: Huang JP]]
[[Category: Li H]]
[[Category: Niu D]]
[[Category: Xie ZZ]]
[[Category: Xu YH]]
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