1uzi: Difference between revisions

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[[Image:1uzi.jpg|left|200px]]
{{Seed}}
[[Image:1uzi.png|left|200px]]


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{{STRUCTURE_1uzi|  PDB=1uzi  |  SCENE=  }}  
{{STRUCTURE_1uzi|  PDB=1uzi  |  SCENE=  }}  


'''C3 EXOENZYME FROM CLOSTRIDIUM BOTULINUM, TETRAGONAL FORM'''
===C3 EXOENZYME FROM CLOSTRIDIUM BOTULINUM, TETRAGONAL FORM===




==Overview==
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C3 exoenzyme from Clostridium botulinum (C3bot1) ADP-ribosylates and thereby inactivates Rho A, B and C GTPases in mammalian cells. The structure of a tetragonal crystal form has been determined by molecular replacement and refined to 1.89 A resolution. It is very similar to the apo structures determined previously from two different monoclinic crystal forms. An objective reassessment of available apo and nucleotide-bound C3bot1 structures indicates that, contrary to a previous report, the protein possesses a rigid core formed largely of beta-strands and that the general flexure that accompanies NAD binding is concentrated in two peripheral lobes. Tetragonal crystals disintegrate in the presence of NAD, most likely because of disruption of essential crystal contacts.
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{{ABSTRACT_PUBMED_15272191}}


==About this Structure==
==About this Structure==
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[[Category: C3]]
[[Category: C3]]
[[Category: Transferase]]
[[Category: Transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 16:33:09 2008''

Revision as of 13:33, 28 July 2008

File:1uzi.png

Template:STRUCTURE 1uzi

C3 EXOENZYME FROM CLOSTRIDIUM BOTULINUM, TETRAGONAL FORM

Template:ABSTRACT PUBMED 15272191

About this Structure

1UZI is a Single protein structure of sequence from Clostridium botulinum. Full crystallographic information is available from OCA.

Reference

C3 exoenzyme from Clostridium botulinum: structure of a tetragonal crystal form and a reassessment of NAD-induced flexure., Evans HR, Holloway DE, Sutton JM, Ayriss J, Shone CC, Acharya KR, Acta Crystallogr D Biol Crystallogr. 2004 Aug;60(Pt 8):1502-5. Epub 2004, Jul 21. PMID:15272191

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