1v33: Difference between revisions

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[[Image:1v33.gif|left|200px]]
{{Seed}}
[[Image:1v33.png|left|200px]]


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{{STRUCTURE_1v33|  PDB=1v33  |  SCENE=  }}  
{{STRUCTURE_1v33|  PDB=1v33  |  SCENE=  }}  


'''Crystal structure of DNA primase from Pyrococcus horikoshii'''
===Crystal structure of DNA primase from Pyrococcus horikoshii===




==Overview==
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BACKGROUND: In chromosomal DNA replication, DNA primase initiates the synthesis of a dinucleotide on a single-stranded template DNA, and elongates it to form a primer RNA for the replicative DNA polymerase. Although the apo-structure of an archaeal primase has been reported, the mechanism of primer synthesis by the eukaryotic-type primase still remains to be elucidated. RESULTS: In this study, we present the crystal structure of the eukaryotic-type DNA primase from the hyperthermophilic archaeon (Pyrococcus horikoshii) with the uridine 5'-triphosphate (UTP). In the present primase-UTP complex, the primase binds the triphosphate moiety of the UTP at the active site, which includes Asp95, Asp97, and Asp280, the essential residues for the nucleotidyl transfer reaction. CONCLUSION: The nucleotide binding geometry in this complex explains the previous biochemical analyses of the eukaryotic primase. Based on the complex structure, we constructed a model between the DNA primase and a primer/template DNA for the primer synthesis. This model facilitates the comprehension of the reported features of DNA primase.
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{{ABSTRACT_PUBMED_14750947}}


==About this Structure==
==About this Structure==
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[[Category: Rsgi]]
[[Category: Rsgi]]
[[Category: Structural genomic]]
[[Category: Structural genomic]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 12:01:06 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 07:19:37 2008''

Revision as of 04:19, 29 July 2008

File:1v33.png

Template:STRUCTURE 1v33

Crystal structure of DNA primase from Pyrococcus horikoshii

Template:ABSTRACT PUBMED 14750947

About this Structure

1V33 is a Single protein structure of sequence from Pyrococcus horikoshii. Full crystallographic information is available from OCA.

Reference

Crystal structure of the Pyrococcus horikoshii DNA primase-UTP complex: implications for the mechanism of primer synthesis., Ito N, Nureki O, Shirouzu M, Yokoyama S, Hanaoka F, Genes Cells. 2003 Dec;8(12):913-23. PMID:14750947

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