1v6d: Difference between revisions

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[[Image:1v6d.gif|left|200px]]
{{Seed}}
[[Image:1v6d.png|left|200px]]


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{{STRUCTURE_1v6d|  PDB=1v6d  |  SCENE=  }}  
{{STRUCTURE_1v6d|  PDB=1v6d  |  SCENE=  }}  


'''The crystal structure of the trypsin complex with synthetic heterochiral peptide'''
===The crystal structure of the trypsin complex with synthetic heterochiral peptide===




==Overview==
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Designed synthetic heterochiral peptides, when added to porcine trypsin, resulted in reduction of enzyme activity. The crystal structure of a complex formed between porcine trypsin and a heterochiral hepta peptide Boc-Pro-DAsp-Aib-Leu-Aib-Leu-Ala-NHMe has been determined at 1.9 A resolution. The hepta peptide does not bind at the active site, but is located in the interstitial region, and interacts with the calcium-binding loop (residues 60-80). The bound peptide interacts with the active site residue Ser195 through an acetate ion, and with Lys 60 mediated by water molecules. The structure, when compared with the other trypsin-peptide complexes, suggests that the flexibility of surface loops, concerted movement of the loops towards the active site, and the interaction of the bound peptide with Lys 60, may be responsible for the reduction in enzyme activity. This study provides a structural evidence for the earlier biochemical observation regarding the role of surface loops in the catalysis of the enzyme.
The line below this paragraph, {{ABSTRACT_PUBMED_15842169}}, adds the Publication Abstract to the page
(as it appears on PubMed at http://www.pubmed.gov), where 15842169 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15842169}}


==About this Structure==
==About this Structure==
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[[Category: Synthetic peptide]]
[[Category: Synthetic peptide]]
[[Category: Trypsin complex]]
[[Category: Trypsin complex]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 12:08:39 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 16:55:04 2008''

Revision as of 13:55, 28 July 2008

File:1v6d.png

Template:STRUCTURE 1v6d

The crystal structure of the trypsin complex with synthetic heterochiral peptide

Template:ABSTRACT PUBMED 15842169

About this Structure

1V6D is a Single protein structure of sequence from Sus scrofa. Full crystallographic information is available from OCA.

Reference

Secondary binding site of trypsin: revealed by crystal structure of trypsin-peptide complex., Shamaladevi N, Pattabhi V, J Biomol Struct Dyn. 2005 Jun;22(6):635-42. PMID:15842169

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