9l6z: Difference between revisions
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==Crystal structure of the L7Ae derivative protein LS12 in RNA-free state== | |||
<StructureSection load='9l6z' size='340' side='right'caption='[[9l6z]], [[Resolution|resolution]] 1.80Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[9l6z]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Archaeoglobus_fulgidus_DSM_4304 Archaeoglobus fulgidus DSM 4304]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9L6Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9L6Z FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9l6z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9l6z OCA], [https://pdbe.org/9l6z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9l6z RCSB], [https://www.ebi.ac.uk/pdbsum/9l6z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9l6z ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/RL7A_ARCFU RL7A_ARCFU] Multifunctional RNA-binding protein that recognizes the K-turn motif in ribosomal RNA, box H/ACA and box C/D sRNAs.<ref>PMID:12560482</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
CS1-LS4 and CS2-LS12 are ultra-high affinity and orthogonal RNA-protein pairs that were identified by PD-SELEX (Phage Display coupled with Systematic Evolution of Ligands by EXponential enrichment). To investigate the molecular basis of the lab-coevolved RNA-RBP pairs, we determined the structures of the CS1-LS4 and CS2-LS12 complexes and the LS12 homodimer in an RNA-free state by X-ray crystallography. The structural analyses revealed that the lab-coevolved RNA-RBPs have acquired unique molecular recognition mechanisms, whereas the overall structures of the RNP complexes were similar to the typical kink-turn RNA-L7Ae complex. The orthogonal RNA-RBP pairs were applied to construct high-performance cell-free riboswitches that regulate translation in response to LS4 or LS12. In addition, by using the orthogonal protein-responsive switches, we generated an AND logic gate that outputs staphylococcal gamma-hemolysin in cell-free system and carried out hemolysis assay and calcein leakage assay using rabbit red blood cells and artificial cells, respectively. | |||
Structural insights into lab-coevolved RNA-RBP pairs and applications of synthetic riboswitches in cell-free system.,Fukunaga K, Teramoto T, Nakashima M, Ohtani T, Katsuki R, Matsuura T, Yokobayashi Y, Kakuta Y Nucleic Acids Res. 2025 Mar 20;53(6):gkaf212. doi: 10.1093/nar/gkaf212. PMID:40119732<ref>PMID:40119732</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 9l6z" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Archaeoglobus fulgidus DSM 4304]] | |||
[[Category: Large Structures]] | |||
[[Category: Fukunaga K]] | |||
[[Category: Kakuta Y]] | |||
[[Category: Nakashima M]] | |||
[[Category: Teramoto T]] | |||
[[Category: Yokobayashi Y]] | |||
Latest revision as of 09:28, 2 April 2025
Crystal structure of the L7Ae derivative protein LS12 in RNA-free state
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