9mxw: Difference between revisions
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==Computationally Designed protein with isopeptide bond dnIPB-1== | |||
<StructureSection load='9mxw' size='340' side='right'caption='[[9mxw]], [[Resolution|resolution]] 2.87Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[9mxw]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9MXW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9MXW FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.87Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9mxw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9mxw OCA], [https://pdbe.org/9mxw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9mxw RCSB], [https://www.ebi.ac.uk/pdbsum/9mxw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9mxw ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Isopeptide bonds (IPBs) horizontal line formed between the amine group of a Lys residue and the carboxamide/carboxy group of Asn/Gln or Asp/Glu horizontal line play essential roles in many biological processes, ranging from cellular signaling and regulation to blood clotting and bacterial pathogenesis. The formation of IPBs is not a spontaneous process and requires enzymatic machinery that provides a specialized active site environment to enable this challenging catalytic reaction. Here we report the de novo design and characterization of two proteins (dnIPB-1 and dnIPB-2) capable of autocatalytic IPB formation. While these designed proteins preserve the key active-site residues of their structural template (the bacterial pilin protein RrgA), they possess less than 31% sequence identity to RrgA. Extensive structural and Ala-scanning analyses indicate that IPB formation requires a solvent-protected core motif composed of several critical residues, yet there is also a large tolerance to different protein topologies and overall protein sizes in terms of accommodating an IPB-forming motif. Notably, the structural insights gained from the study of dnIPB-1 and dnIPB-2 also guided the redesign of an initially failed construct (dnIPB-3) and enabled it to form an IPB, highlighting the value of de novo design in examining sequence-structure-function relationships not explored in natural evolution. Our study highlights the versatility of IPBs as designable elements which can be used to construct functional proteins or protein-based materials with enhanced chemical, thermal, and mechanical stabilities. | |||
De Novo Design of Proteins for Autocatalytic Isopeptide Bond Formation.,Srisantitham S, Walker AL, Markel U, Tezcan FA J Am Chem Soc. 2025 Mar 26. doi: 10.1021/jacs.5c03319. PMID:40138671<ref>PMID:40138671</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 9mxw" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Synthetic construct]] | |||
[[Category: Srisantitham S]] | |||
[[Category: Tezcan FA]] | |||
Latest revision as of 08:14, 9 April 2025
Computationally Designed protein with isopeptide bond dnIPB-1
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