9m7u: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older edit
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,331,613 edits
m Protected "9m7u" [edit=sysop:move=sysop]
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,331,613 edits
No edit summary
 
Line 1: Line 1:
'''Unreleased structure'''


The entry 9m7u is ON HOLD
==At S1+tRNA trimer==
<StructureSection load='9m7u' size='340' side='right'caption='[[9m7u]], [[Resolution|resolution]] 3.18&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9m7u]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9M7U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9M7U FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.18&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9m7u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9m7u OCA], [https://pdbe.org/9m7u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9m7u RCSB], [https://www.ebi.ac.uk/pdbsum/9m7u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9m7u ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
S-type anion channel homologs (SLAH) are widely expressed in various plant tissues and play a key role in anion transport, which is crucial for plant adaptation to both biotic and abiotic stresses. In this study, we employ cryo-electron microscopy (cryo-EM) to analyze four SLAH channel complexes from Arabidopsis thaliana: the homotrimeric SLAH3 channel, the 2SLAH1 + SLAH3+tRNA complex, the 1SLAH1 + 2SLAH3 complex, and the 3SLAH1+tRNA complex. Critically, our studies reveal that tRNA directly binds to and occupies the intracellular entrance of the SLAH1 homotrimer and the 2SLAH1 + SLAH3 heterocomplex. Electrophysiological experiments confirm tRNA's role as a potent inhibitory regulatory subunit: RNase-mediated tRNA degradation robustly activates SLAH1 currents, while targeted mutagenesis of SLAH1 tRNA-interacting residues phenocopy this activation and enhanced ABA-induced stomatal closure. Combining with structural biology, electrophysiology, and biochemistry, we comprehensively examine the key residues in SLAH1 and SLAH3 that are responsible for the anion permeation. This mechanistic advancement provides a deeper understanding of the molecular basis for plant stress tolerance and identifies specific molecular targets for future engineering crops.


Authors: Zhang, S.S.
Structural insights into the coordinated regulation of the SLAH family in Arabidopsis thaliana.,Zhang S, Huang X, Wang X, Qi B, Yang K, Liu C, Li R, Chen X, Yi J, Yin J, Chen M, Liu B, Fan J, Liu T, Hao Z, Cui K, Xiao N, Song Y, Li Y, Wu D, Qi T, Mao J, Zhang B, Yang M, Xie J, Liu Z Nat Commun. 2025 Dec 17;17(1):585. doi: 10.1038/s41467-025-67283-6. PMID:41407702<ref>PMID:41407702</ref>


Description: At S1+tRNA trimer
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
[[Category: Zhang, S.S]]
<div class="pdbe-citations 9m7u" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Arabidopsis thaliana]]
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Zhang SS]]
Retrieved from "https://proteopedia.org/9m7u"