1yx4: Difference between revisions
New page: left|200px<br /> <applet load="1yx4" size="450" color="white" frame="true" align="right" spinBox="true" caption="1yx4" /> '''Structure of S5a bound to monoubiquitin pro... |
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[[Image:1yx4.gif|left|200px]]<br /> | [[Image:1yx4.gif|left|200px]]<br /><applet load="1yx4" size="350" color="white" frame="true" align="right" spinBox="true" | ||
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'''Structure of S5a bound to monoubiquitin provides a model for polyubiquitin recognition'''<br /> | '''Structure of S5a bound to monoubiquitin provides a model for polyubiquitin recognition'''<br /> | ||
==Overview== | ==Overview== | ||
Ubiquitin is a key regulatory molecule in diverse cellular events. How | Ubiquitin is a key regulatory molecule in diverse cellular events. How cells determine the outcome of ubiquitylation remains unclear; however, a likely determinant is the specificity of ubiquitin receptor proteins for polyubiquitin chains of certain length and linkage. Proteasome subunit S5a contains two ubiquitin-interacting motifs (UIMs) through which it recruits ubiquitylated substrates to the proteasome for their degradation. Here, we report the structure of S5a (196-306) alone and complexed with two monoubiquitin molecules. This construct contains the two UIMs of S5a and we reveal their different ubiquitin-binding mechanisms and provide a rationale for their unique specificities for different ubiquitin-like domains. Furthermore, we provide direct evidence that S5a (196-306) binds either K63-linked or K48-linked polyubiquitin, and in both cases prefers longer chains. On the basis of these results we present a model for how S5a and other ubiquitin-binding proteins recognize polyubiquitin. | ||
==About this Structure== | ==About this Structure== | ||
1YX4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | 1YX4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YX4 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Walters, K | [[Category: Walters, K J.]] | ||
[[Category: Wang, Q.]] | [[Category: Wang, Q.]] | ||
[[Category: Young, P.]] | [[Category: Young, P.]] | ||
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[[Category: uim]] | [[Category: uim]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:09:59 2008'' | ||
Revision as of 14:10, 21 February 2008
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Structure of S5a bound to monoubiquitin provides a model for polyubiquitin recognition
Overview
Ubiquitin is a key regulatory molecule in diverse cellular events. How cells determine the outcome of ubiquitylation remains unclear; however, a likely determinant is the specificity of ubiquitin receptor proteins for polyubiquitin chains of certain length and linkage. Proteasome subunit S5a contains two ubiquitin-interacting motifs (UIMs) through which it recruits ubiquitylated substrates to the proteasome for their degradation. Here, we report the structure of S5a (196-306) alone and complexed with two monoubiquitin molecules. This construct contains the two UIMs of S5a and we reveal their different ubiquitin-binding mechanisms and provide a rationale for their unique specificities for different ubiquitin-like domains. Furthermore, we provide direct evidence that S5a (196-306) binds either K63-linked or K48-linked polyubiquitin, and in both cases prefers longer chains. On the basis of these results we present a model for how S5a and other ubiquitin-binding proteins recognize polyubiquitin.
About this Structure
1YX4 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structure of S5a bound to monoubiquitin provides a model for polyubiquitin recognition., Wang Q, Young P, Walters KJ, J Mol Biol. 2005 May 6;348(3):727-39. PMID:15826667
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