9lw7: Difference between revisions
From Proteopedia
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
==Midsection of bacteriophage Mycofy1 mature head (C5 symmetry)== | |||
<StructureSection load='9lw7' size='340' side='right'caption='[[9lw7]], [[Resolution|resolution]] 3.52Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[9lw7]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycolicibacterium_phage_Mycofy1 Mycolicibacterium phage Mycofy1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9LW7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9LW7 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.52Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9lw7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9lw7 OCA], [https://pdbe.org/9lw7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9lw7 RCSB], [https://www.ebi.ac.uk/pdbsum/9lw7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9lw7 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/Q854Z2_9CAUD Q854Z2_9CAUD] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Mycobacteriophages show promise in treating antibiotic-resistant mycobacterial infections. Here, we isolated Mycofy1, a mycobacteriophage, using M. smegmatis as a host. Cryo-EM analysis revealed that Mycofy1 possesses a prolate head and a long non-contractile tail. We determined structures of its head, head-to-tail interface, terminator, and tail tube to resolutions of approximately 3.5 A. Unexpectedly, we identified two distinct types of prolate head structures, exhibiting a 36-degree relative rotation in the top cap region. Additionally, the head-to-tail interface demonstrated flexibility. Our structures provide the first high-resolution cryo-EM data of a mycobacteriophage with a prolate head, as well as detailed structural information of the head-to-tail interface and head-proximal tail region in this phage group. These findings advance our understanding of assembly mechanisms in tailed bacteriophages. | |||
Cryo-EM reveals structural diversity in prolate-headed mycobacteriophage Mycofy1.,Li X, Shao Q, Li L, Xie L, Ruan Z, Fang Q J Mol Biol. 2025 Apr 3:169126. doi: 10.1016/j.jmb.2025.169126. PMID:40187685<ref>PMID:40187685</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 9lw7" style="background-color:#fffaf0;"></div> | ||
[[Category: | == References == | ||
[[Category: | <references/> | ||
[[Category: | __TOC__ | ||
[[Category: | </StructureSection> | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: Mycolicibacterium phage Mycofy1]] | |||
[[Category: Fang Q]] | |||
[[Category: Li L]] | |||
[[Category: Li X]] | |||
[[Category: Ruan Z]] | |||
[[Category: Shao Q]] | |||
[[Category: Xie L]] | |||
Latest revision as of 11:31, 16 April 2025
Midsection of bacteriophage Mycofy1 mature head (C5 symmetry)
| ||||||||||||
