1vfg: Difference between revisions

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[[Image:1vfg.gif|left|200px]]
{{Seed}}
[[Image:1vfg.png|left|200px]]


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{{STRUCTURE_1vfg|  PDB=1vfg  |  SCENE=  }}  
{{STRUCTURE_1vfg|  PDB=1vfg  |  SCENE=  }}  


'''Crystal structure of tRNA nucleotidyltransferase complexed with a primer tRNA and an incoming ATP analog'''
===Crystal structure of tRNA nucleotidyltransferase complexed with a primer tRNA and an incoming ATP analog===




==Overview==
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The 3'-terminal CCA nucleotide sequence (positions 74-76) of transfer RNA is essential for amino acid attachment and interaction with the ribosome during protein synthesis. The CCA sequence is synthesized de novo and/or repaired by a template-independent RNA polymerase, 'CCA-adding enzyme', using CTP and ATP as substrates. Despite structural and biochemical studies, the mechanism by which the CCA-adding enzyme synthesizes the defined sequence without a nucleic acid template remains elusive. Here we present the crystal structure of Aquifex aeolicus CCA-adding enzyme, bound to a primer tRNA lacking the terminal adenosine and an incoming ATP analogue, at 2.8 A resolution. The enzyme enfolds the acceptor T helix of the tRNA molecule. In the catalytic pocket, C75 is adjacent to ATP, and their base moieties are stacked. The complementary pocket for recognizing C74-C75 of tRNA forms a 'protein template' for the penultimate two nucleotides, mimicking the nucleotide template used by template-dependent polymerases. These results are supported by systematic analyses of mutants. Our structure represents the 'pre-insertion' stage of selecting the incoming nucleotide and provides the structural basis for the mechanism underlying template-independent RNA polymerization.
The line below this paragraph, {{ABSTRACT_PUBMED_15295603}}, adds the Publication Abstract to the page
(as it appears on PubMed at http://www.pubmed.gov), where 15295603 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15295603}}


==About this Structure==
==About this Structure==
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[[Category: Structural genomic]]
[[Category: Structural genomic]]
[[Category: Transferase]]
[[Category: Transferase]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 12:28:48 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 07:33:24 2008''

Revision as of 04:33, 29 July 2008

File:1vfg.png

Template:STRUCTURE 1vfg

Crystal structure of tRNA nucleotidyltransferase complexed with a primer tRNA and an incoming ATP analog

Template:ABSTRACT PUBMED 15295603

About this Structure

1VFG is a Single protein structure of sequence from Aquifex aeolicus. Full crystallographic information is available from OCA.

Reference

Structural basis for template-independent RNA polymerization., Tomita K, Fukai S, Ishitani R, Ueda T, Takeuchi N, Vassylyev DG, Nureki O, Nature. 2004 Aug 5;430(7000):700-4. PMID:15295603

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