Sandbox Reserved 1846: Difference between revisions
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=== Catalytic Triad === | === Catalytic Triad === | ||
LCC catalyzes the breakdown of PET using a classic serine hydrolase mechanism involving a catalytic triad of Ser165, His242, and Asp210. The reaction begins when His242 deprotonates Ser165, which activates it as a nucleophile. Ser165 then attacks the carbonyl carbon of an ester bond in the PET polymer. This forms a tetrahedral intermediate. This intermediate is stabilized by an oxyanion hole. The intermediate collapses; one product is released and an acyl-enzyme intermediate is formed. A water molecule, activated by His242, then attacks the acyl-enzyme, releasing the second product and resetting the enzyme’s active site. | |||
[[Image:Mechanism_(1).jpeg|1100 px]] | [[Image:Mechanism_(1).jpeg|1100 px]] | ||
