Sandbox Reserved 1852: Difference between revisions
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==Introduction== | ==Introduction== | ||
The Diels Alderase aims to catalyze the Diels-Alder reaction for use in synthetic organic chemistry. Specifically, the enzyme surpasses uncatalyzed reactions by generating a product that is entirely [https://en.wikipedia.org/wiki/Stereoselectivity#:~:text=In%20chemistry%2C%20stereoselectivity%20is%20the,of%20a%20pre%2Dexisting%20one. stereoselective] for the 3R,4S endo form. The Diels-Alderase was built using ''de novo'' enzyme design, using computational modeling and refinement through collaborative problem-solving from online users. The first generation Diels-Alderase was made using the [https://en.wikipedia.org/wiki/Rosetta@home Rosetta] computational design program, where a potential active site was built and tested against a library of scaffold proteins. Later, as the active site was perfected, future generations of the Diels-Alderase were made using an online protein folding game called [https://en.wikipedia.org/wiki/Foldit Foldit,] where players competed to improve binding efficiency by completing various challenges.<ref name="Eiben"/> | The Diels Alderase aims to catalyze the Diels-Alder reaction for use in synthetic organic chemistry. Specifically, the enzyme surpasses uncatalyzed reactions by generating a product that is entirely [https://en.wikipedia.org/wiki/Stereoselectivity#:~:text=In%20chemistry%2C%20stereoselectivity%20is%20the,of%20a%20pre%2Dexisting%20one. stereoselective] for the 3R,4S endo form. The Diels-Alderase was built using ''de novo'' enzyme design, using computational modeling and refinement through collaborative problem-solving from online users. The first generation Diels-Alderase was made using the [https://en.wikipedia.org/wiki/Rosetta@home Rosetta] computational design program, where a potential active site was built and tested against a library of scaffold proteins. Later, as the active site was perfected, future generations of the Diels-Alderase were made using an online protein folding game called [https://en.wikipedia.org/wiki/Foldit Foldit,] where players competed to improve binding efficiency by completing various challenges.<ref name="Eiben"/> | ||
