Sandbox323: Difference between revisions

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== Overview ==
== Overview ==
=== General Structure and Origins ===


4Q7Q exists as a homodimer Quaternary structure.<ref name="Sequence">4Q7Q. Protein Database, 2014.</ref> Analyzing primary and Quaternary structures of 4Q7Q with SPRITE and Chimera revealed two chains identical in both shape and sequence<ref name="SPRITE">Nadzirin, N.; Gardiner, E.; Willett, P.; Artymiuk, P. J.; Firdaus-Raih, M. 2012. SPRITE and ASSAM: web servers for side chain 3D-motif searching in protein structures. Nucleic Acids Res., 40(Web Server Issue), W380-6.</ref> Each chain is 266 residues long, and the entire complex has a molecular weight of approximately 58.5 kDa.<ref name="Sequence" />.
4Q7Q exists as a homodimer Quaternary structure.<ref name="Sequence">4Q7Q. Protein Database, 2014.</ref> Analyzing primary and Quaternary structures of 4Q7Q with SPRITE and Chimera revealed two chains identical in both shape and sequence<ref name="SPRITE">Nadzirin, N.; Gardiner, E.; Willett, P.; Artymiuk, P. J.; Firdaus-Raih, M. 2012. SPRITE and ASSAM: web servers for side chain 3D-motif searching in protein structures. Nucleic Acids Res., 40(Web Server Issue), W380-6.</ref> Each chain is 266 residues long, and the entire complex has a molecular weight of approximately 58.5 kDa.<ref name="Sequence" />.
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[[Image:4Q7QABSequence.png|300px|left|thumb| Primary Sequences of the A and B chains of 4Q7Q.<ref name="SPRITE" />.]]
[[Image:4Q7QABSequence.png|300px|left|thumb| Primary Sequences of the A and B chains of 4Q7Q.<ref name="SPRITE" />.]]


4Q7Q proteins originate from bacterial species.G,H InterPro search results show how nearly every enzyme with similar sequencing to 4Q7Q is found in various bacteria, with a notable exception to eukaryotes.D Additionally, the PDB entry for 4Q7Q notes how it potentially can be found in Chitinophaga pinensis, a gram-negative bacterial species which can degrade chitin.G,H
4Q7Q proteins primarily appear in bacterial species.<ref name="Origin">Rio, T. G. D.; et al. Complete genome sequence of Chitinophaga pinensis type strain (UQM 2034). Stand. Genomic. Sci., 2010, 2(1), 87-95. https://pmc.ncbi.nlm.nih.gov/articles/PMC3035255/</ref>. Research reveals how nearly every enzyme with similar sequencing to 4Q7Q is found in bacterium, with a slight exception to eukaryotes.<ref name="SGNH">SGNH hydrolase superfamily. InterPro, 2017. https://www.ebi.ac.uk/interpro/entry/InterPro/IPR036514/</ref> Additionally, the PDB entry for 4Q7Q notes how it potentially can be found in Chitinophaga pinensis, a gram-negative bacterial species which can degrade chitin.<ref name="Origin" />. <ref name="GDSL">Rio, T. G. D.; et al. Complete genome sequence of Chitinophaga pinensis type strain (UQM 2034). Stand. Genomic. Sci., 2010, 2(1), 87-95. https://pmc.ncbi.nlm.nih.gov/articles/PMC3035255/ </ref>.


== Family and Superfamily ==
=== Family and Superfamily ===


Research shows that 4Q7Q is a member of the SGNH Hydrolase protein super family. BLAST and InterPro both suggested 4Q7Q’s inclusion in this family, and the known conserved residues seen from SPRITE analysis—Serine, Glycine, Asparagine, and Histidine—line up with those observed throughout this family.D,E Notably, this superfamily is also referred to as the GDSL Hydrolase superfamily.D,E
Research shows that 4Q7Q is a member of the SGNH Hydrolase protein super family. BLAST and InterPro both suggested 4Q7Q’s inclusion in this family, and the known conserved residues seen from SPRITE analysis—Serine, Glycine, Asparagine, and Histidine—line up with those observed throughout this family.D,E Notably, this superfamily is also referred to as the GDSL Hydrolase superfamily.D,E
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