Sandbox2O14: Difference between revisions
From Proteopedia
Jump to navigationJump to search
Matthew Lamb (talk | contribs) No edit summary |
Matthew Lamb (talk | contribs) No edit summary |
||
| Line 46: | Line 46: | ||
== Figure 4 == | == Figure 4 == | ||
[[Image: | [[Image:1_30mg1stSMALL.jpg]] | ||
Protein test using 10µL of protein from elution and 30mg of PNPP and 3mL of PNPP solution. Measuring absorbance at 405M. | Protein test using 10µL of protein from elution and 30mg of PNPP and 3mL of PNPP solution. Measuring absorbance at 405M. | ||
== Figure 5 == | == Figure 5 == | ||
[[Image:1_30mg1stSMALL.jpg]] | |||
Protein test using 10µL of protein from elution 2 and 30mg of PNPP and 3mL of PNPP solution. Measuring absorbance at 405M. | Protein test using 10µL of protein from elution 2 and 30mg of PNPP and 3mL of PNPP solution. Measuring absorbance at 405M. | ||
== Figure 6 == | == Figure 6 == | ||
[[Image:1_30mg1stSMALL.jpg]] | |||
Protein test using 10µL of protein from elution 1 and 30mg of PNPP and 3mL of PNPP solution, changing the pH to about 5.60. Measuring absorbance at 405M. | Protein test using 10µL of protein from elution 1 and 30mg of PNPP and 3mL of PNPP solution, changing the pH to about 5.60. Measuring absorbance at 405M. | ||
| Line 61: | Line 63: | ||
== Figure 8 == | == Figure 8 == | ||
[[Image:1_30mg1stSMALL.jpg]] | |||
Protein test using 10µL of protein from elution 1 and 3mg of PNPP and 3mL of PNPP solution. Measuring absorbance at 405M. | Protein test using 10µL of protein from elution 1 and 3mg of PNPP and 3mL of PNPP solution. Measuring absorbance at 405M. | ||
== Figure 9 == | == Figure 9 == | ||
[[Image:1_30mg1stSMALL.jpg]] | |||
Protein test using 10µL of protein from elution 1 and 3mg of PNPP and 3mL of PNPP solution and 1 drop of HCL with a pH of about 10.50. Measuring absorbance at 405M. | Protein test using 10µL of protein from elution 1 and 3mg of PNPP and 3mL of PNPP solution and 1 drop of HCL with a pH of about 10.50. Measuring absorbance at 405M. | ||
== Figure 10 == | == Figure 10 == | ||
[[Image:1_30mg1stSMALL.jpg]] | |||
Protein test using 10µL of protein from elution 1 and 10mg of PNPA and 10mL of PNPP solution and 1 drop of HCL with a pH of about 10.50. Measuring absorbance at 405M. | Protein test using 10µL of protein from elution 1 and 10mg of PNPA and 10mL of PNPP solution and 1 drop of HCL with a pH of about 10.50. Measuring absorbance at 405M. | ||
| Line 72: | Line 77: | ||
== Figure 11 == | == Figure 11 == | ||
[[Image:1_30mg1stSMALL.jpg]] | |||
Protein test using 10µL of protein from elution 1 and 15mg of PNPA and 3mL of PNPP solution. Measuring absorbance at 405M. | Protein test using 10µL of protein from elution 1 and 15mg of PNPA and 3mL of PNPP solution. Measuring absorbance at 405M. | ||
== Figure 12 == | == Figure 12 == | ||
[[Image:1_30mg1stSMALL.jpg]] | |||
Protein test with increasing protein solution of 5µL every minute from elution 1 with 15mg of PNPA and 3mL PNPP solution. The pH of the solution is 8.35 and it was measuring absorbance at 405M. | Protein test with increasing protein solution of 5µL every minute from elution 1 with 15mg of PNPA and 3mL PNPP solution. The pH of the solution is 8.35 and it was measuring absorbance at 405M. | ||
