Sandbox323: Difference between revisions
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[[Image:Absorbancelongph7cold.png|800px|left|]] | [[Image:Absorbancelongph7cold.png|800px|left|]] | ||
50uL of purified protein from the first elution was added to 3mL of cold 1mg/mL PNPA at pH=7.30. Chloride ion cofactor was provided by the 2M HCl. A change in absorbance at 428.7nm over time was noted. The time range of the run did not produce a linear response. The absorbance increased for the first ten minutes and plateaued. The time 0 to 10 minutes was used to create the third figure. | 50uL of purified protein from the first elution was added to 3mL of cold 1mg/mL PNPA at pH=7.30. Chloride ion cofactor was provided by the 2M HCl. A change in absorbance at 428.7nm over time was noted. The time range of the run did not produce a linear response. The absorbance increased for the first ten minutes and plateaued. The time 0 to 10 minutes was used to create the third figure with a more refined linear range. | ||
[[Image:Absorbancecoldph7try.png|800px|left|]] | |||
Using Beer-Lambert law and accepting the approximation of the Molar Extinction Coefficient for PNPA hydrolysis at 428.7nm as the same value as | |||
[[Image:Concentrationcoldph7.jpg|800px|left|]] | [[Image:Concentrationcoldph7.jpg|800px|left|]] | ||
