Sandbox323: Difference between revisions
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[[Image:Concentrationcoldmolarabsorb12000ph7.png|500px|left|]] | [[Image:Concentrationcoldmolarabsorb12000ph7.png|500px|left|]] | ||
Absorbance values can be transformed to units of concentration via the Beer-Lambert law. We must accept the approximation of the Molar Extinction Coefficient for PNPA hydrolysis at 428.7nm as 12000 M^-1 cm^-1. An example calculation is supplied in the table. | Absorbance values can be transformed to units of concentration via the Beer-Lambert law. We must accept the approximation of the Molar Extinction Coefficient for PNPA hydrolysis at 428.7nm as 12000 M^-1 cm^-1. An example calculation is supplied in the table. Graphing time versus concentration and determining the slope of the line yields the enzyme's velocity in M/min. 1mg/mL of PNPA is saturating conditions which implies the Vmax is also the slope. The reaction volume total times Vmax yields Units of Enzyme Activity. This value can be used as a relative comparison tool for enzyme performance in given conditions. | ||
[[Image:Icedactivitytableph7.png|500px|left|]] | |||
[[Image:Tablebeerlaw.png|500px|left|]] | [[Image:Tablebeerlaw.png|500px|left|]] | ||
The cold 4 degrees C reaction was changed to room temperature 20 degrees C. All other conditions remained constant to evaluate the effect of temperature on enzyme. | |||
