Sandbox323: Difference between revisions
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A linear equation of y=9.275x10^-7x+1x10^-4 with a coefficient of determination of 0.8492 was calculated using linear regression. | A linear equation of y=9.275x10^-7x+1x10^-4 with a coefficient of determination of 0.8492 was calculated using linear regression. | ||
[[Image:Concentrationcoldmolarabsorb12000ph7.png|500px|left|]] | [[Image:Concentrationcoldmolarabsorb12000ph7.png|500px|left|thumb|]] | ||
Absorbance values can be transformed to units of concentration via the Beer-Lambert law. We must accept the approximation of the Molar Extinction Coefficient for PNPA hydrolysis at 428.7nm as 12000 M^-1 cm^-1. An example calculation is supplied in the table. Graphing time versus concentration and determining the slope of the line yields the enzyme's velocity in M/min. 1mg/mL of PNPA is saturating conditions which implies the Vmax is also the slope. The reaction volume total times Vmax yields Units of Enzyme Activity. This value can be used as a relative comparison tool for enzyme performance in given conditions. | Absorbance values can be transformed to units of concentration via the Beer-Lambert law. We must accept the approximation of the Molar Extinction Coefficient for PNPA hydrolysis at 428.7nm as 12000 M^-1 cm^-1. An example calculation is supplied in the table. Graphing time versus concentration and determining the slope of the line yields the enzyme's velocity in M/min. 1mg/mL of PNPA is saturating conditions which implies the Vmax is also the slope. The reaction volume total times Vmax yields Units of Enzyme Activity. This value can be used as a relative comparison tool for enzyme performance in given conditions. | ||
[[Image:Icedactivitytableph7.png| | [[Image:Icedactivitytableph7.png|700px|left|thumb|Enzyme Activity at neutral pH at cold temperature with Chloride cofactor]] | ||
[[Image:Tablebeerlaw.png|700px|left|]] | [[Image:Tablebeerlaw.png|700px|left|thumb|Beer-Lambert Law sample calculation]] | ||
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[[Image:Sigfigroomtempenzymeactivity.png| | [[Image:Sigfigroomtempenzymeactivity.png|700px|left|thumb|Enzyme Activity at neutral pH at room temperature with Chloride cofactor ]] | ||
