Sandbox323: Difference between revisions
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=== Substrate and Hydrolysis of Substrate === | === Substrate and Hydrolysis of Substrate === | ||
p-nitrophenyl acetate (PNPA) is an aromatic compound with an ester linkage. In the presence of an esterase, the compound is susceptible to cleavage. The products are acetate and p-nitrophenolate anion (PNP). PNP is yellow in color so reaction progress can be monitored by measuring the absorption peak at 405nm using a spectrophotometer. | p-nitrophenyl acetate (PNPA) is an aromatic compound with an ester linkage. In the presence of an esterase, the compound is susceptible to cleavage. The products are acetate and p-nitrophenolate anion (PNP). PNP is yellow in color so reaction progress can be monitored by measuring the absorption peak at 405nm using a spectrophotometer. | ||
[[Image:PNPA.png|400px|right|The reaction used to analyze the activity of our protein<ref name="BasilAssay">Protein Activity Assays Student Module. BASIL. | |||
https://docs.google.com/document/d/1G-H0tskmSVRhC0qsDau-7-EzUcUYN8kBUHFZyBStYcs/edit?tab=t.0 (accessed 04/28/25).</ref>]] | |||
=== Spectrophotometry === | === Spectrophotometry === | ||
