Sandbox323: Difference between revisions
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[[Image:Tablebeerlaw.png|1000px|left|thumb|Beer-Lambert Law sample calculation.]] | [[Image:Tablebeerlaw.png|1000px|left|thumb|Beer-Lambert Law sample calculation.]] | ||
[[Image:Concentrationcoldmolarabsorb12000ph7.png|400px|left|thumb|A trendline showing the | [[Image:Concentrationcoldmolarabsorb12000ph7.png|400px|left|thumb|A trendline showing the relationship between time and product concentration from the data gathered from the PNPA+Protein solution with pH = 7, sodium chloride, and a cold environment.]] | ||
Absorbance values can be transformed to units of concentration via the Beer-Lambert law. We must accept the approximation of the Molar Extinction Coefficient for PNPA hydrolysis at 428.7nm as 12000 M^-1 cm^-1. An example calculation is supplied in the table. Graphing time versus concentration and determining the slope of the line yields the enzyme's velocity in M/min. 1mg/mL of PNPA is saturating conditions which implies the Vmax is also the slope. The reaction volume total times Vmax yields Units of Enzyme Activity. This value can be used as a relative comparison tool for enzyme performance in given conditions. | Absorbance values can be transformed to units of concentration via the Beer-Lambert law. We must accept the approximation of the Molar Extinction Coefficient for PNPA hydrolysis at 428.7nm as 12000 M^-1 cm^-1. An example calculation is supplied in the table. Graphing time versus concentration and determining the slope of the line yields the enzyme's velocity in M/min. 1mg/mL of PNPA is saturating conditions which implies the Vmax is also the slope. The reaction volume total times Vmax yields Units of Enzyme Activity. This value can be used as a relative comparison tool for enzyme performance in given conditions. | ||
