Sandbox Reserved 1846: Difference between revisions
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The ICCG variant (F243I/D238C/S283C/Y127G) showed a 1.22-fold higher specific activity than wild-type LCC, with a melting temperature increase of +9.3°C. It achieved 90% PET depolymerization in 9.3 hours at 72°C.<ref name="Tournier"/> | The ICCG variant (F243I/D238C/S283C/Y127G) showed a 1.22-fold higher specific activity than wild-type LCC, with a melting temperature increase of +9.3°C. It achieved 90% PET depolymerization in 9.3 hours at 72°C.<ref name="Tournier"/> | ||
The WCCG variant (F243W/D238C/S283C/Y127G) had specific activity | The WCCG variant (F243W/D238C/S283C/Y127G) had specific activity slightly lower than ICCG, but showed even greater thermostability, with a melting temperature increase of +10.1°C. It reached 90% PET depolymerization in 10.5 hours at 72°C.<ref name="Tournier"/> | ||
Other stabilizing mutations, such as T96M, N246D, and N246M, were also tested but | Other stabilizing mutations, such as T96M, N246D, and N246M, were also tested, but excluded as they were not part of the top-performing mutant (ICCG), and therefore omitted for clarity.<ref name="Tournier"/> | ||
</StructureSection> | </StructureSection> | ||
