1z9e: Difference between revisions

Line 1:

[[Image:1z9e.gif|left|200px]]<br />

[[Image:1z9e.gif|left|200px]]<br /><applet load="1z9e" size="350" color="white" frame="true" align="right" spinBox="true"

<applet load="1z9e" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

caption="1z9e" />

'''Solution structure of the HIV-1 integrase-binding domain in LEDGF/p75'''<br />

==Overview==

Lens epithelium-derived growth factor (LEDGF)/p75 is the dominant binding, partner of HIV-1 integrase (IN) in human cells. We have determined the NMR, structure of the integrase-binding domain (IBD) in LEDGF and identified, amino acid residues essential for the interaction. The IBD is a compact, right-handed bundle composed of five alpha-helices. Based on folding, topology, the IBD is structurally related to a diverse family of, alpha-helical proteins that includes eukaryotic translation initiation, factor eIF4G and karyopherin-beta. LEDGF residues essential for the, interaction with IN were localized to interhelical loop regions of the, bundle structure. Interaction-defective IN mutants were previously shown, to cripple replication although they retained catalytic function. The, initial structure determination of a host cell factor that tightly binds, to a retroviral enzyme lays the groundwork for understanding enzyme-host, interactions important for viral replication.

Lens epithelium-derived growth factor (LEDGF)/p75 is the dominant binding partner of HIV-1 integrase (IN) in human cells. We have determined the NMR structure of the integrase-binding domain (IBD) in LEDGF and identified amino acid residues essential for the interaction. The IBD is a compact right-handed bundle composed of five alpha-helices. Based on folding topology, the IBD is structurally related to a diverse family of alpha-helical proteins that includes eukaryotic translation initiation factor eIF4G and karyopherin-beta. LEDGF residues essential for the interaction with IN were localized to interhelical loop regions of the bundle structure. Interaction-defective IN mutants were previously shown to cripple replication although they retained catalytic function. The initial structure determination of a host cell factor that tightly binds to a retroviral enzyme lays the groundwork for understanding enzyme-host interactions important for viral replication.

==About this Structure==

1Z9E is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1Z9E OCA].

1Z9E is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z9E OCA].

==Reference==

Line 18:

Line 17:

[[Category: Maertens, G.]]

[[Category: Rahman, S.]]

[[Category: Sun, Z.Y.J.]]

[[Category: Sun, Z Y.J.]]

[[Category: Wagner, G.]]

[[Category: heat repeat-like]]

[[Category: ledgf]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Mon Nov 12 20~~:31:~~26 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:13:25 2008''

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools

@@ Line 1: / Line 1: @@
-[[Image:1z9e.gif|left|200px]]<br />
+[[Image:1z9e.gif|left|200px]]<br /><applet load="1z9e" size="350" color="white" frame="true" align="right" spinBox="true"
-<applet load="1z9e" size="450" color="white" frame="true" align="right" spinBox="true"
 caption="1z9e" />
 '''Solution structure of the HIV-1 integrase-binding domain in LEDGF/p75'''<br />
 ==Overview==
-Lens epithelium-derived growth factor (LEDGF)/p75 is the dominant binding, partner of HIV-1 integrase (IN) in human cells. We have determined the NMR, structure of the integrase-binding domain (IBD) in LEDGF and identified, amino acid residues essential for the interaction. The IBD is a compact, right-handed bundle composed of five alpha-helices. Based on folding, topology, the IBD is structurally related to a diverse family of, alpha-helical proteins that includes eukaryotic translation initiation, factor eIF4G and karyopherin-beta. LEDGF residues essential for the, interaction with IN were localized to interhelical loop regions of the, bundle structure. Interaction-defective IN mutants were previously shown, to cripple replication although they retained catalytic function. The, initial structure determination of a host cell factor that tightly binds, to a retroviral enzyme lays the groundwork for understanding enzyme-host, interactions important for viral replication.
+Lens epithelium-derived growth factor (LEDGF)/p75 is the dominant binding partner of HIV-1 integrase (IN) in human cells. We have determined the NMR structure of the integrase-binding domain (IBD) in LEDGF and identified amino acid residues essential for the interaction. The IBD is a compact right-handed bundle composed of five alpha-helices. Based on folding topology, the IBD is structurally related to a diverse family of alpha-helical proteins that includes eukaryotic translation initiation factor eIF4G and karyopherin-beta. LEDGF residues essential for the interaction with IN were localized to interhelical loop regions of the bundle structure. Interaction-defective IN mutants were previously shown to cripple replication although they retained catalytic function. The initial structure determination of a host cell factor that tightly binds to a retroviral enzyme lays the groundwork for understanding enzyme-host interactions important for viral replication.
 ==About this Structure==
-Z9E is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1Z9E OCA].
+Z9E is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z9E OCA].
 ==Reference==
@@ Line 18: / Line 17: @@
 [[Category: Maertens, G.]]
 [[Category: Rahman, S.]]
-[[Category: Sun, Z.Y.J.]]
+[[Category: Sun, Z Y.J.]]
 [[Category: Wagner, G.]]
 [[Category: heat repeat-like]]
 [[Category: ledgf]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 20:31:26 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:13:25 2008''