9r46: Difference between revisions
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==Spitrobot-2 advances time-resolvedcryo-trapping crystallography to under 25 ms: Xylose isomerase bound with glucose (25 ms soaking)== | |||
<StructureSection load='9r46' size='340' side='right'caption='[[9r46]], [[Resolution|resolution]] 2.10Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[9r46]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_pseudogriseolus_group Streptomyces pseudogriseolus group]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9R46 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9R46 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9r46 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9r46 OCA], [https://pdbe.org/9r46 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9r46 RCSB], [https://www.ebi.ac.uk/pdbsum/9r46 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9r46 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/XYLA_STRRU XYLA_STRRU] Involved in D-xylose catabolism. | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
We previously introduced the spitrobot, a protein crystal plunging system that enables reaction quenching via cryo-trapping with a time resolution in the millisecond range. Here we present the next generation, spitrobot-2, as an integrated benchtop device. User-friendliness has been improved by semi-automatic sample exchange. Moreover, a fully automated shutter shields the liquid nitrogen from the humidified environment, improving sample integrity. Most importantly, the cryo-trapping delay time has been reduced to 23 ms, making spitrobot-2 twice as fast as the previous generation. This further expands the number of target systems that can be addressed by cryo-trapping time-resolved crystallography. Using 12 crystal structures of three independent model systems, we demonstrate successful cryo-trapping via observation of conformational changes and ligand binding within 25 ms. These improvements increase the convenient access to cryo-trapping, time-resolved X-ray crystallography empowering the MX community with efficient tools to advance research in structural biology. | |||
Spitrobot-2 advances time-resolved cryo-trapping crystallography to under 25 ms.,Spiliopoulou M, Hatton CE, Kollewe M, Leimkohl JP, Schikora H, Tellkamp F, Mehrabi P, Schulz EC Commun Chem. 2025 Nov 20;8(1):363. doi: 10.1038/s42004-025-01784-9. PMID:41266557<ref>PMID:41266557</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 9r46" style="background-color:#fffaf0;"></div> | ||
[[Category: | == References == | ||
[[Category: Mehrabi | <references/> | ||
[[Category: | __TOC__ | ||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Streptomyces pseudogriseolus group]] | |||
[[Category: Hatton CE]] | |||
[[Category: Mehrabi P]] | |||
[[Category: Schulz EC]] | |||
[[Category: Spiliopoulou M]] | |||
Latest revision as of 19:34, 4 December 2025
Spitrobot-2 advances time-resolvedcryo-trapping crystallography to under 25 ms: Xylose isomerase bound with glucose (25 ms soaking)
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