1w8f: Difference between revisions

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[[Image:1w8f.gif|left|200px]]
{{Seed}}
[[Image:1w8f.png|left|200px]]


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{{STRUCTURE_1w8f|  PDB=1w8f  |  SCENE=  }}  
{{STRUCTURE_1w8f|  PDB=1w8f  |  SCENE=  }}  


'''PSEUDOMONAS AERUGINOSA LECTIN II (PA-IIL) COMPLEXED WITH LACTO-N-NEO-FUCOPENTAOSE V(LNPFV)'''
===PSEUDOMONAS AERUGINOSA LECTIN II (PA-IIL) COMPLEXED WITH LACTO-N-NEO-FUCOPENTAOSE V(LNPFV)===




==Overview==
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One of the mechanisms contributing to the protection by breast-feeding of the newborn against enteric diseases is related to the ability of human milk oligosaccharides to prevent the attachment of pathogenic bacteria to the duodenual epithelium. Indeed, a variety of fucosylated oligosaccharides, specific to human milk, form part of the innate immune system. In the present study, we demonstrate the specific blocking of PA-IIL, a fucose-binding lectin of the human pathogen Pseudomonas aeruginosa, by milk oligosaccharides. Two fucosylated epitopes, Lewis a and 3-fucosyl-lactose (Lewis x glucose analogue) bind to the lectin with dissociation constants of 2.2x10(-7) M and 3.6x10(-7) M respectively. Thermodynamic studies indicate that these interactions are dominated by enthalpy. The entropy contribution is slightly favourable when binding to fucose and to the highest-affinity ligand, Lewis a. The high-resolution X-ray structures of two complexes of PA-IIL with milk oligosaccharides allow the precise determination of the conformation of a trisaccharide and a pentasaccharide. The different types of interaction between the oligosaccharides and the protein involve not only hydrogen bonding, but also calcium- and water-bridged contacts, allowing a rationalization of the thermodynamic data. This study provides important structural information about compounds that could be of general application in new therapeutic strategies against bacterial infections.
The line below this paragraph, {{ABSTRACT_PUBMED_15790314}}, adds the Publication Abstract to the page
(as it appears on PubMed at http://www.pubmed.gov), where 15790314 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15790314}}


==About this Structure==
==About this Structure==
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[[Category: Wimmerova, M.]]
[[Category: Wimmerova, M.]]
[[Category: Lectin,pentassacharide,cystic firosis,pseudomonas aeruginosa,infection of lung]]
[[Category: Lectin,pentassacharide,cystic firosis,pseudomonas aeruginosa,infection of lung]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 13:18:04 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 15:18:51 2008''

Revision as of 12:18, 28 July 2008

File:1w8f.png

Template:STRUCTURE 1w8f

PSEUDOMONAS AERUGINOSA LECTIN II (PA-IIL) COMPLEXED WITH LACTO-N-NEO-FUCOPENTAOSE V(LNPFV)

Template:ABSTRACT PUBMED 15790314

About this Structure

1W8F is a Single protein structure of sequence from Pseudomonas aeruginosa. Full crystallographic information is available from OCA.

Reference

Structural basis for the interaction between human milk oligosaccharides and the bacterial lectin PA-IIL of Pseudomonas aeruginosa., Perret S, Sabin C, Dumon C, Pokorna M, Gautier C, Galanina O, Ilia S, Bovin N, Nicaise M, Desmadril M, Gilboa-Garber N, Wimmerova M, Mitchell EP, Imberty A, Biochem J. 2005 Jul 15;389(Pt 2):325-32. PMID:15790314

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