9vc9: Difference between revisions
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==Cryo-EM structure of Shewanella decolorationis ComEC== | |||
<StructureSection load='9vc9' size='340' side='right'caption='[[9vc9]], [[Resolution|resolution]] 2.77Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[9vc9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Shewanella_decolorationis Shewanella decolorationis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9VC9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9VC9 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.77Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AV0:(2~{R},3~{R},4~{S},5~{S},6~{R})-2-[(2~{R},3~{S},4~{R},5~{R},6~{R})-6-[2-decyl-2-[[(2~{R},3~{R},4~{R},5~{S},6~{R})-6-(hydroxymethyl)-5-[(2~{R},3~{R},4~{S},5~{S},6~{R})-6-(hydroxymethyl)-3,4,5-tris(oxidanyl)oxan-2-yl]oxy-3,4-bis(oxidanyl)oxan-2-yl]oxymethyl]dodecoxy]-2-(hydroxymethyl)-4,5-bis(oxidanyl)oxan-3-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol'>AV0</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9vc9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9vc9 OCA], [https://pdbe.org/9vc9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9vc9 RCSB], [https://www.ebi.ac.uk/pdbsum/9vc9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9vc9 ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Natural transformation is one of the major pathways of horizontal gene transfer in bacteria, enabling the acquisition of extracellular DNA and its integration into the host genome. ComEC is a membrane protein responsible for DNA translocation in this process, yet its precise function and structure have remained elusive. Here, we report cryo-electron microscopy structures of ComEC in DNA-free, single-stranded DNA (ssDNA)-bound, and double-stranded DNA (dsDNA)-bound forms, together with biochemical analyses. These structures reveal that ComEC cleaves one strand of dsDNA at its extracellular domain and guides the remaining strand into a positively charged pore formed within the membrane domain. These findings provide a structural basis for the long-hypothesized roles of ComEC in both DNA processing and translocation across the inner membrane during natural transformation. | |||
Structural basis for DNA processing and membrane translocation by ComEC in natural transformation.,Hirano H, Tsuji N, Chiba S, Nureki O Science. 2026 Apr 16;392(6795):311-316. doi: 10.1126/science.aea3485. Epub 2026 , Apr 16. PMID:41990170<ref>PMID:41990170</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 9vc9" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Shewanella decolorationis]] | |||
[[Category: Hirano H]] | |||
[[Category: Nureki O]] | |||
Latest revision as of 08:27, 29 April 2026
Cryo-EM structure of Shewanella decolorationis ComEC
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