9k7g: Difference between revisions

Latest revision as of 08:08, 29 April 2026

Structure of PfENT1 in complex with GSK3

Structural highlights

9k7g is a 1 chain structure with sequence from Plasmodium falciparum 3D7. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Electron Microscopy, Resolution 3.57Å
Ligands:	A1EE2
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

ENT1_PLAF7 Sodium-independent nucleoside and nucleobase transporter with a broad substrate specificity (PubMed:10744765, PubMed:10861212, PubMed:16751273, PubMed:18639591, PubMed:36977719). Plays a key role in the utilization of host purine sources by P.falciparum during intraerythrocytic development enabling parasite growth in the presence of physiological concentrations of adenosine, inosine, guanine, guanosine, xanthine and hypoxanthine (PubMed:16751273, PubMed:18639591). Essential for parasite transition from ring to trophozoite or from trophozoite to schizont stage but not for erythrocyte invasion by merozoites (PubMed:16751273).^[1] ^[2] ^[3] ^[4] ^[5]

References

↑ Carter NS, Ben Mamoun C, Liu W, Silva EO, Landfear SM, Goldberg DE, Ullman B. Isolation and functional characterization of the PfNT1 nucleoside transporter gene from Plasmodium falciparum. J Biol Chem. 2000 Apr 7;275(14):10683-91. PMID:10744765 doi:10.1074/jbc.275.14.10683
↑ Parker MD, Hyde RJ, Yao SY, McRobert L, Cass CE, Young JD, McConkey GA, Baldwin SA. Identification of a nucleoside/nucleobase transporter from Plasmodium falciparum, a novel target for anti-malarial chemotherapy. Biochem J. 2000 Jul 1;349(Pt 1):67-75. PMID:10861212 doi:10.1042/0264-6021:3490067
↑ El Bissati K, Zufferey R, Witola WH, Carter NS, Ullman B, Ben Mamoun C. The plasma membrane permease PfNT1 is essential for purine salvage in the human malaria parasite Plasmodium falciparum. Proc Natl Acad Sci U S A. 2006 Jun 13;103(24):9286-91. PMID:16751273 doi:10.1073/pnas.0602590103
↑ El Bissati K, Downie MJ, Kim SK, Horowitz M, Carter N, Ullman B, Ben Mamoun C. Genetic evidence for the essential role of PfNT1 in the transport and utilization of xanthine, guanine, guanosine and adenine by Plasmodium falciparum. Mol Biochem Parasitol. 2008 Oct;161(2):130-9. PMID:18639591 doi:10.1016/j.molbiopara.2008.06.012
↑ Wang C, Yu L, Zhang J, Zhou Y, Sun B, Xiao Q, Zhang M, Liu H, Li J, Li J, Luo Y, Xu J, Lian Z, Lin J, Wang X, Zhang P, Guo L, Ren R, Deng D. Structural basis of the substrate recognition and inhibition mechanism of Plasmodium falciparum nucleoside transporter PfENT1. Nat Commun. 2023 Mar 28;14(1):1727. PMID:36977719 doi:10.1038/s41467-023-37411-1

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Carter NS, Ben Mamoun C, Liu W, Silva EO, Landfear SM, Goldberg DE, Ullman B. Isolation and functional characterization of the PfNT1 nucleoside transporter gene from Plasmodium falciparum. J Biol Chem. 2000 Apr 7;275(14):10683-91. PMID:10744765 doi:10.1074/jbc.275.14.10683

[2] Parker MD, Hyde RJ, Yao SY, McRobert L, Cass CE, Young JD, McConkey GA, Baldwin SA. Identification of a nucleoside/nucleobase transporter from Plasmodium falciparum, a novel target for anti-malarial chemotherapy. Biochem J. 2000 Jul 1;349(Pt 1):67-75. PMID:10861212 doi:10.1042/0264-6021:3490067

[3] El Bissati K, Zufferey R, Witola WH, Carter NS, Ullman B, Ben Mamoun C. The plasma membrane permease PfNT1 is essential for purine salvage in the human malaria parasite Plasmodium falciparum. Proc Natl Acad Sci U S A. 2006 Jun 13;103(24):9286-91. PMID:16751273 doi:10.1073/pnas.0602590103

[4] El Bissati K, Downie MJ, Kim SK, Horowitz M, Carter N, Ullman B, Ben Mamoun C. Genetic evidence for the essential role of PfNT1 in the transport and utilization of xanthine, guanine, guanosine and adenine by Plasmodium falciparum. Mol Biochem Parasitol. 2008 Oct;161(2):130-9. PMID:18639591 doi:10.1016/j.molbiopara.2008.06.012

[5] Wang C, Yu L, Zhang J, Zhou Y, Sun B, Xiao Q, Zhang M, Liu H, Li J, Li J, Luo Y, Xu J, Lian Z, Lin J, Wang X, Zhang P, Guo L, Ren R, Deng D. Structural basis of the substrate recognition and inhibition mechanism of Plasmodium falciparum nucleoside transporter PfENT1. Nat Commun. 2023 Mar 28;14(1):1727. PMID:36977719 doi:10.1038/s41467-023-37411-1

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[5]

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 9k7g is ON HOLD  until Paper Publication
+==Structure of PfENT1 in complex with GSK3==
+<StructureSection load='9k7g' size='340' side='right'caption='[[9k7g]], [[Resolution|resolution]] 3.57&Aring;' scene=''>
-Authors:
+== Structural highlights ==
+<table><tr><td colspan='2'>[[9k7g]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Plasmodium_falciparum_3D7 Plasmodium falciparum 3D7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9K7G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9K7G FirstGlance]. <br>
-Description:
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.57&#8491;</td></tr>
-[[Category: Unreleased Structures]]
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A1EE2:~{N}-cyclopropyl-2-[[3-(2-oxidanylidenepyrrolidin-1-yl)phenyl]carbonylamino]benzamide'>A1EE2</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9k7g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9k7g OCA], [https://pdbe.org/9k7g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9k7g RCSB], [https://www.ebi.ac.uk/pdbsum/9k7g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9k7g ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/ENT1_PLAF7 ENT1_PLAF7] Sodium-independent nucleoside and nucleobase transporter with a broad substrate specificity (PubMed:10744765, PubMed:10861212, PubMed:16751273, PubMed:18639591, PubMed:36977719). Plays a key role in the utilization of host purine sources by P.falciparum during intraerythrocytic development enabling parasite growth in the presence of physiological concentrations of adenosine, inosine, guanine, guanosine, xanthine and hypoxanthine (PubMed:16751273, PubMed:18639591). Essential for parasite transition from ring to trophozoite or from trophozoite to schizont stage but not for erythrocyte invasion by merozoites (PubMed:16751273).<ref>PMID:10744765</ref> <ref>PMID:10861212</ref> <ref>PMID:16751273</ref> <ref>PMID:18639591</ref> <ref>PMID:36977719</ref>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Plasmodium falciparum 3D7]]
+[[Category: Chen XY]]
+[[Category: Deng D]]
+[[Category: Li JL]]
+[[Category: Wang X]]

9k7g: Difference between revisions

Latest revision as of 08:08, 29 April 2026

Structure of PfENT1 in complex with GSK3

Structural highlights

Function

References

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