9p6r: Difference between revisions
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==Structure of lysozyme-N,N',N"-triacetylchitotriose complex determined using REyes for automated MicroED== | |||
<StructureSection load='9p6r' size='340' side='right'caption='[[9p6r]], [[Resolution|resolution]] 2.20Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[9p6r]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9P6R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9P6R FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron crystallography, [[Resolution|Resolution]] 2.2Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9p6r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9p6r OCA], [https://pdbe.org/9p6r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9p6r RCSB], [https://www.ebi.ac.uk/pdbsum/9p6r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9p6r ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
In the hands of experts, microcrystal electron diffraction (microED, a 3D ED method) is a powerful tool for structural chemistry and chemical discovery. To expand the accessibility and utility of microED, we introduce Reciprocal Eyes (REyes), an autonomous and intelligent platform (available for academic use) that combines diffraction-based particle selection with real-time data processing to deliver crystal structures without human intervention. REyes spatially maps diffraction signal and autonomously selects crystallites of interest, relying on lattice-quality metrics to acquire and index high-resolution data sets from diverse compounds. Tested on four different transmission electron microscopes (TEMs), it consistently yields preliminary ab initio structural solutions from single crystallites of materials, peptides, metal complexes, natural products (NPs), and proteins. | |||
Spatially Aware Diffraction Mapping Enables Fully Autonomous MicroED.,Eremin DB, Jha KK, Delgadillo DA, Zhang H, Foxman SH, Johnson SN, Vlahakis NW, Cascio D, Lavallo V, Rodriguez JA, Nelson HM J Am Chem Soc. 2025 Nov 19;147(46):42299-42310. doi: 10.1021/jacs.5c10751. Epub , 2025 Nov 10. PMID:41211926<ref>PMID:41211926</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 9p6r" style="background-color:#fffaf0;"></div> | ||
[[Category: Nelson | == References == | ||
[[Category: Rodriguez | <references/> | ||
[[Category: | __TOC__ | ||
</StructureSection> | |||
[[Category: Gallus gallus]] | |||
[[Category: Large Structures]] | |||
[[Category: Eremin D]] | |||
[[Category: Nelson HM]] | |||
[[Category: Rodriguez JA]] | |||
[[Category: Vlahakis NW]] | |||
