1wbp: Difference between revisions

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[[Image:1wbp.~~gif~~|left|200px]]

[[Image:1wbp.png|left|200px]]

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~~'''~~SRPK1 BOUND TO 9MER DOCKING MOTIF PEPTIDE~~'''~~

===SRPK1 BOUND TO 9MER DOCKING MOTIF PEPTIDE===

~~==Overview==~~

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The ~~arginine-serine (RS)-rich domain of the SR protein ASF/SF2 is phosphorylated by SR protein kinases (SRPKs) and Clk/Sty kinases. However~~, ~~the mode of phosphorylation by these kinases and their coordination in the biological regulation of ASF/SF2 is unknown. Here~~, ~~we report~~ the ~~crystal structure of an active fragment of human SRPK1 bound~~ to ~~a peptide derived from an SR protein. This structure led us to identify a docking motif in ASF~~/~~SF2. We find that this docking motif restricts phosphorylation of ASF~~/~~SF2 by SRPK1 to the N-terminal part of the RS domain - a property essential for its assembly into nuclear speckles~~. ~~We further show that Clk/Sty causes release of ASF/SF2 from speckles by phosphorylating the C-terminal part of its RS domain~~. ~~These results suggest that the docking motif of ASF/SF2 is a key regulatory element for sequential phosphorylation by SRPK1 and Clk/Sty and, thus~~, is ~~essential for its subcellular localization~~.

The line below this paragraph, {{ABSTRACT_PUBMED_16209947}}, adds the Publication Abstract to the page

(as it appears on PubMed at http://www.pubmed.gov), where 16209947 is the PubMed ID number.

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==About this Structure==

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[[Category: Srpk]]

[[Category: Transferase]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sat May 3~~ 13:25:52 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 13:23:06 2008''

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-[[Image:1wbp.gif|left|200px]]
+{{Seed}}
+[[Image:1wbp.png|left|200px]]
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 {{STRUCTURE_1wbp|  PDB=1wbp  |  SCENE=  }}
-'''SRPK1 BOUND TO 9MER DOCKING MOTIF PEPTIDE'''
+===SRPK1 BOUND TO 9MER DOCKING MOTIF PEPTIDE===
-==Overview==
+<!--
-The arginine-serine (RS)-rich domain of the SR protein ASF/SF2 is phosphorylated by SR protein kinases (SRPKs) and Clk/Sty kinases. However, the mode of phosphorylation by these kinases and their coordination in the biological regulation of ASF/SF2 is unknown. Here, we report the crystal structure of an active fragment of human SRPK1 bound to a peptide derived from an SR protein. This structure led us to identify a docking motif in ASF/SF2. We find that this docking motif restricts phosphorylation of ASF/SF2 by SRPK1 to the N-terminal part of the RS domain - a property essential for its assembly into nuclear speckles. We further show that Clk/Sty causes release of ASF/SF2 from speckles by phosphorylating the C-terminal part of its RS domain. These results suggest that the docking motif of ASF/SF2 is a key regulatory element for sequential phosphorylation by SRPK1 and Clk/Sty and, thus, is essential for its subcellular localization.
+The line below this paragraph, {{ABSTRACT_PUBMED_16209947}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 16209947 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_16209947}}
 ==About this Structure==
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 [[Category: Srpk]]
 [[Category: Transferase]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 13:25:52 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 13:23:06 2008''