1wc7: Difference between revisions

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[[Image:1wc7.gif|left|200px]]
{{Seed}}
[[Image:1wc7.png|left|200px]]


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{{STRUCTURE_1wc7|  PDB=1wc7  |  SCENE=  }}  
{{STRUCTURE_1wc7|  PDB=1wc7  |  SCENE=  }}  


'''FAB FRAGMENT OF PLP-DEPENDENT CATALYTIC ANTIBODY 15A9 IN COMPLEX WITH PHOSPHOPYRIDOXYL-L-ALANINE'''
===FAB FRAGMENT OF PLP-DEPENDENT CATALYTIC ANTIBODY 15A9 IN COMPLEX WITH PHOSPHOPYRIDOXYL-L-ALANINE===




==Overview==
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The purification, crystallization and structure determination at 2.3 A resolution of the complex of the pyridoxal-5'-phosphate (PLP) dependent catalytic antibody 15A9 with a phosphopyridoxyl-L-alanine (PPL-L-alanine) substrate analogue are described. The crystal belongs to space group P2(1), with two molecules in the asymmetric unit related by non-crystallographic symmetry. The unit-cell parameters are a = 63.5, b = 81.7, c = 79.3 A and beta is fortuitously 90 degrees . Refinement of the structure converged at unacceptably high R factors. Although the traditional analysis of intensity distribution did not indicate twinning, pseudomerohedral twinning was revealed by a newer test based on local intensity differences [Padilla &amp; Yeates (2003), Acta Cryst. D59, 1124-1130]. When the potential twinning operator was included in SHELX, the structure could be satisfactorily refined with a twinning fraction of 0.46, indicating a nearly perfect hemihedrally twinned crystal. One of the active sites is occupied by the phosphopyridoxyl-L-alanine ligand, while one iodide ion mimics the cofactor phosphate group in the other. Four other iodide ions are present in the structure: two are involved in specific intermolecular contacts and two dictate the conformation of the CDRH3 loop in each molecule.
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{{ABSTRACT_PUBMED_15805602}}


==About this Structure==
==About this Structure==
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[[Category: Pyridoxal-phosphate]]
[[Category: Pyridoxal-phosphate]]
[[Category: Transamination]]
[[Category: Transamination]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 13:27:08 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 20:28:18 2008''

Revision as of 17:28, 27 July 2008

File:1wc7.png

Template:STRUCTURE 1wc7

FAB FRAGMENT OF PLP-DEPENDENT CATALYTIC ANTIBODY 15A9 IN COMPLEX WITH PHOSPHOPYRIDOXYL-L-ALANINE

Template:ABSTRACT PUBMED 15805602

About this Structure

1WC7 is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.

Reference

Structure of a pseudomerohedrally twinned monoclinic crystal form of a pyridoxal phosphate-dependent catalytic antibody., Golinelli-Pimpaneau B, Acta Crystallogr D Biol Crystallogr. 2005 Apr;61(Pt 4):472-6. Epub 2005, Mar 24. PMID:15805602

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