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| [[Image:1wd3.gif|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_1wd3| PDB=1wd3 | SCENE= }} | | {{STRUCTURE_1wd3| PDB=1wd3 | SCENE= }} |
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| '''Crystal structure of arabinofuranosidase'''
| | ===Crystal structure of arabinofuranosidase=== |
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| ==Overview==
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| As the first known structures of a glycoside hydrolase family 54 (GH54) enzyme, we determined the crystal structures of free and arabinose-complex forms of Aspergillus kawachii IFO4308 alpha-l-arabinofuranosidase (AkAbfB). AkAbfB comprises two domains: a catalytic domain and an arabinose-binding domain (ABD). The catalytic domain has a beta-sandwich fold similar to those of clan-B glycoside hydrolases. ABD has a beta-trefoil fold similar to that of carbohydrate-binding module (CBM) family 13. However, ABD shows a number of characteristics distinctive from those of CBM family 13, suggesting that it could be classified into a new CBM family. In the arabinose-complex structure, one of three arabinofuranose molecules is bound to the catalytic domain through many interactions. Interestingly, a disulfide bond formed between two adjacent cysteine residues recognized the arabinofuranose molecule in the active site. From the location of this arabinofuranose and the results of a mutational study, the nucleophile and acid/base residues were determined to be Glu(221) and Asp(297), respectively. The other two arabinofuranose molecules are bound to ABD. The O-1 atoms of the two arabinofuranose molecules bound at ABD are both pointed toward the solvent, indicating that these sites can both accommodate an arabinofuranose side-chain moiety linked to decorated arabinoxylans.
| | The line below this paragraph, {{ABSTRACT_PUBMED_15292273}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 15292273 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_15292273}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Beta-sandwich]] | | [[Category: Beta-sandwich]] |
| [[Category: Beta-trefoil]] | | [[Category: Beta-trefoil]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 13:29:28 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 17:02:13 2008'' |
