1wij: Difference between revisions

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[[Image:1wij.jpg|left|200px]]
{{Seed}}
[[Image:1wij.png|left|200px]]


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{{STRUCTURE_1wij|  PDB=1wij  |  SCENE=  }}  
{{STRUCTURE_1wij|  PDB=1wij  |  SCENE=  }}  


'''Solution Structure of the DNA-Binding Domain of Ethylene-Insensitive3-Like3'''
===Solution Structure of the DNA-Binding Domain of Ethylene-Insensitive3-Like3===




==Overview==
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Ethylene-insensitive3 (EIN3) and EIN3-like (EIL) proteins are essential transcription factors in the ethylene signaling of higher plants. The EIN3/EIL proteins bind to the promoter regions of the downstream genes and regulate their expression. The location of the DNA-binding domain (DBD) in the primary structure was unclear, since the proteins show no sequence similarity to other known DBDs. Here, we identify the major DBD of an EIN3/EIL protein, Arabidopsis thaliana EIL3, containing a key mutational site for DNA binding and signaling (ein3-3 site), and determine its solution structure by NMR spectroscopy. The structure consists of five alpha-helices, possessing a novel fold dissimilar to known DBD structures. By a chemical-shift perturbation analysis, a region including the ein3-3 site is suggested to be involved in DNA binding.
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{{ABSTRACT_PUBMED_15811366}}


==About this Structure==
==About this Structure==
1WIJ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WIJ OCA].  
1WIJ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WIJ OCA].  


==Reference==
==Reference==
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[[Category: Rsgi]]
[[Category: Rsgi]]
[[Category: Structural genomic]]
[[Category: Structural genomic]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 13:43:27 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 21:01:52 2008''

Revision as of 18:01, 28 July 2008

File:1wij.png

Template:STRUCTURE 1wij

Solution Structure of the DNA-Binding Domain of Ethylene-Insensitive3-Like3

Template:ABSTRACT PUBMED 15811366

About this Structure

1WIJ is a Single protein structure of sequence from Arabidopsis thaliana. Full experimental information is available from OCA.

Reference

Solution structure of the major DNA-binding domain of Arabidopsis thaliana ethylene-insensitive3-like3., Yamasaki K, Kigawa T, Inoue M, Yamasaki T, Yabuki T, Aoki M, Seki E, Matsuda T, Tomo Y, Terada T, Shirouzu M, Tanaka A, Seki M, Shinozaki K, Yokoyama S, J Mol Biol. 2005 Apr 29;348(2):253-64. PMID:15811366

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