1wkv: Difference between revisions

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[[Image:1wkv.gif|left|200px]]
{{Seed}}
[[Image:1wkv.png|left|200px]]


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{{STRUCTURE_1wkv|  PDB=1wkv  |  SCENE=  }}  
{{STRUCTURE_1wkv|  PDB=1wkv  |  SCENE=  }}  


'''Crystal structure of O-phosphoserine sulfhydrylase'''
===Crystal structure of O-phosphoserine sulfhydrylase===




==Overview==
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O-Phosphoserine sulfhydrylase is a new enzyme found in a hyperthermophilic archaeon, Aeropyrum pernix K1. This enzyme catalyzes a novel cysteine synthetic reaction from O-phospho-l-serine and sulfide. The crystal structure of the enzyme was determined at 2.0A resolution using the method of multi-wavelength anomalous dispersion. A monomer consists of three domains, including an N-terminal domain with a new alpha/beta fold. The topology folds of the middle and C-terminal domains were similar to those of the O-acetylserine sulfhydrylase-A from Salmonella typhimurium and the cystathionine beta-synthase from human. The cofactor, pyridoxal 5'-phosphate, is bound in a cleft between the middle and C-terminal domains through a covalent linkage to Lys127. Based on the structure determined, O-phospho-l-serine could be rationally modeled into the active site of the enzyme. An enzyme-substrate complex model and a mutation experiment revealed that Arg297, unique to hyperthermophilic archaea, is one of the most crucial residues for O-phosphoserine sulfhydrylation activity. There are more hydrophobic areas and less electric charges at the dimer interface, compared to the S.typhimurium O-acetylserine sulfhydrylase.
The line below this paragraph, {{ABSTRACT_PUBMED_16005886}}, adds the Publication Abstract to the page
(as it appears on PubMed at http://www.pubmed.gov), where 16005886 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16005886}}


==About this Structure==
==About this Structure==
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[[Category: Homodimer]]
[[Category: Homodimer]]
[[Category: Open alpha/beta folding]]
[[Category: Open alpha/beta folding]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 13:49:15 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 23:08:47 2008''

Revision as of 20:08, 28 July 2008

File:1wkv.png

Template:STRUCTURE 1wkv

Crystal structure of O-phosphoserine sulfhydrylase

Template:ABSTRACT PUBMED 16005886

About this Structure

1WKV is a Single protein structure of sequence from Aeropyrum pernix. Full crystallographic information is available from OCA.

Reference

Three-dimensional structure of a new enzyme, O-phosphoserine sulfhydrylase, involved in l-cysteine biosynthesis by a hyperthermophilic archaeon, Aeropyrum pernix K1, at 2.0A resolution., Oda Y, Mino K, Ishikawa K, Ataka M, J Mol Biol. 2005 Aug 12;351(2):334-44. PMID:16005886

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