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| {{STRUCTURE_1wlw| PDB=1wlw | SCENE= }} | | {{STRUCTURE_1wlw| PDB=1wlw | SCENE= }} |
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| '''Congerin II Y16S single mutant'''
| | ===Congerin II Y16S single mutant=== |
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| ==Overview==
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| The thermostability of the conger eel galectin, congerin II, was improved by in vitro evolutionary protein engineering. Two rounds of random PCR mutagenesis and selection experiments increased the congerin II thermostability to a level comparative to its naturally thermostable isoform, congerin I. The crystal structures of the most thermostable double mutant, Y16S/T88I, and the related single mutants, Y16S and T88I, were determined at 2.0 angstroms, 1.8 angstroms, and 1.6 angstroms resolution, respectively. The exclusion of two interior water molecules by the Thr88Ile mutation, and the relief of adjacent conformational stress by the Tyr16Ser mutation were the major contributions to the thermostability. These features in the congerin II mutants are similar to those observed in congerin I. The natural evolution of congerin genes, with the K(A)/K(S) ratio of 2.6, was accelerated under natural selection pressures. The thermostabilizing selection pressure artificially applied to congerin II mimicked the implied natural pressure on congerin I. The results showed that the artificial pressure made congerin II partially reproduce the natural evolution of congerin I. | | The line below this paragraph, {{ABSTRACT_PUBMED_15740748}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 15740748 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_15740748}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Mutant]] | | [[Category: Mutant]] |
| [[Category: Thermostability]] | | [[Category: Thermostability]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 13:51:22 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 23:27:04 2008'' |
