1wni: Difference between revisions

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[[Image:1wni.gif|left|200px]]
{{Seed}}
[[Image:1wni.png|left|200px]]


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{{STRUCTURE_1wni|  PDB=1wni  |  SCENE=  }}  
{{STRUCTURE_1wni|  PDB=1wni  |  SCENE=  }}  


'''Crystal Structure of H2-Proteinase'''
===Crystal Structure of H2-Proteinase===




==Overview==
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The crystal structure of the zinc-protease, H2-proteinase, isolated from the venom of Trimeresurus flavoviridis has been determined. The crystallographic R factor is 0.176 for 10,635 reflections with Fobs &gt; 2sigma(Fobs) in the 8.0 to 2.2 Angstrom resolution range. The enzyme has two domains with a cleft in which a catalytic zinc atom is located. The N-terminal domain is composed of four helices around a central five-stranded beta-sheet. The irregularly folded C-terminal domain contains one helix and two disulfide bridges. These two domains are linked by a disulfide bridge. In the zinc environment, the catalytic zinc atom forms a distorted tetrahedral coordination with three histidines and one catalytic water molecule, and the methionine-containing turn is structurally conserved. These are distinctive features of the metzincins, one of the zinc metalloprotease superfamilies. The entire structure shows good agreement with that of two Crotalus snake venom proteases, adamalysin II and atrolysin C. The H2-proteinase, however, contains no structural calcium ions, and differences of disulfide configurations and the coordination of the catalytic water molecule exist as compared with the other two proteases.
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==About this Structure==
==About this Structure==
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==Reference==
==Reference==
Crystal structure of H2-proteinase from the venom of Trimeresurus flavoviridis., Kumasaka T, Yamamoto M, Moriyama H, Tanaka N, Sato M, Katsube Y, Yamakawa Y, Omori-Satoh T, Iwanaga S, Ueki T, J Biochem. 1996 Jan;119(1):49-57. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8907175 8907175]
Crystal structure of H2-proteinase from the venom of Trimeresurus flavoviridis., Kumasaka T, Yamamoto M, Moriyama H, Tanaka N, Sato M, Katsube Y, Yamakawa Y, Omori-Satoh T, Iwanaga S, Ueki T, J Biochem. 1996 Jan;119(1):49-57. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8907175 8907175]
Crystallization and preliminary X-ray study of H2-proteinase from the venom of Trimeresurus flavoviridis., Kumasaka T, Takeya H, Yamamoto M, Yamakawa Y, Omori-Satoh T, Moriyama H, Tanaka N, Sato M, Katsube Y, Iwanaga S, J Biochem. 1995 May;117(5):929-30. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8586633 8586633]
[[Category: Single protein]]
[[Category: Single protein]]
[[Category: Trimerelysin II]]
[[Category: Trimerelysin II]]
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[[Category: Snake venom]]
[[Category: Snake venom]]
[[Category: Zinc]]
[[Category: Zinc]]
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