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| {{STRUCTURE_1wno| PDB=1wno | SCENE= }} | | {{STRUCTURE_1wno| PDB=1wno | SCENE= }} |
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| '''Crystal structure of a native chitinase from Aspergillus fumigatus YJ-407'''
| | ===Crystal structure of a native chitinase from Aspergillus fumigatus YJ-407=== |
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| ==Overview==
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| Chitinase hydrolyzes chitin, a linear polymer of beta-1,4-linked N-acetylglucosamine (NAG), and plays a variety of roles in the biological world. In addition to endo- and exo-hydrolytic activities, transglycosyl activity has also been observed in the extracellular chitinase (afCHI) from the airborne saprophytic fungi Aspergillus fumigatus YJ-407. Crystals of this native chitinase have been grown at 291 K using PEG 3350 as a precipitant. The diffraction data from the crystal extend to 1.7 A resolution at BSRF, China. The crystal belongs to space group P2(1)2(1)2(1), with unit-cell parameters a = 95.7, b = 100.5, c = 134.3 A. The presence of two molecules per asymmetric unit gives a crystal volume per protein mass (V(M)) of 3.6 A(3) Da(-1) and a solvent content of 65% by volume. A full set of X-ray diffraction data was collected to 2.1 A resolution.
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| ==About this Structure== | | ==About this Structure== |
| 1WNO is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Aspergillus_fumigatus Aspergillus fumigatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WNO OCA]. | | 1WNO is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Aspergillus_fumigatus Aspergillus fumigatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WNO OCA]. |
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| ==Reference== | | ==Reference== |
| Crystallization and preliminary crystallographic analysis of a native chitinase from the fungal pathogen Aspergillus fumigatus YJ-407., Hu H, Wang G, Yang H, Zhou J, Mo L, Yang K, Jin C, Jin C, Rao Z, Acta Crystallogr D Biol Crystallogr. 2004 May;60(Pt 5):939-40. Epub 2004, Apr 21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15103145 15103145]
| | <ref group="xtra">PMID:15103145</ref><references group="xtra"/> |
| [[Category: Aspergillus fumigatus]] | | [[Category: Aspergillus fumigatus]] |
| [[Category: Chitinase]] | | [[Category: Chitinase]] |
| [[Category: Single protein]]
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| [[Category: Hu, H.]] | | [[Category: Hu, H.]] |
| [[Category: Jin, C.]] | | [[Category: Jin, C.]] |
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| [[Category: Zhou, J.]] | | [[Category: Zhou, J.]] |
| [[Category: Eight-stranded beta/alpha-barrel]] | | [[Category: Eight-stranded beta/alpha-barrel]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 13:55:19 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 18:12:22 2009'' |
