9xys: Difference between revisions
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==Crystal structure of juvenile hormone acid methyltransferase CfJHAMT in complex with SAH and juvenile hormone III acid== | |||
<StructureSection load='9xys' size='340' side='right'caption='[[9xys]], [[Resolution|resolution]] 1.77Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[9xys]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Choristoneura_fumiferana Choristoneura fumiferana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9XYS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9XYS FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.77Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=JH3:METHYL+(2E,6E)-9-[(2R)-3,3-DIMETHYLOXIRAN-2-YL]-3,7-DIMETHYLNONA-2,6-DIENOATE'>JH3</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9xys FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9xys OCA], [https://pdbe.org/9xys PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9xys RCSB], [https://www.ebi.ac.uk/pdbsum/9xys PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9xys ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Juvenile hormone acid methyltransferase (JHAMT) is an enzyme involved in the biosynthesis of juvenile hormone (JH) in insects, catalyzing the methylation of farnesoic acid and JH acids to produce active JHs. Given its important role in JH biosynthesis, JHAMT has attracted significant interest as a potential target for pest control strategies. Inhibiting JHAMT activity could disrupt normal JH production, leading to developmental abnormalities and reduced reproductive success in pest species. We have determined the crystal structure of a JHAMT from the spruce budworm Choristoneura fumiferana (CfJHAMT) in complex with the cofactor product S-adenosyl-L-homocysteine (SAH) and the substrate juvenile hormone acid III, at a resolution of 1.77 A, and in the presence of SAH alone. Structural and biochemical analyses, supported by site-directed mutagenesis, revealed key residues involved in cofactor and substrate recognition. A proximity-based catalytic mechanism is proposed wherein critical interactions position the substrate and cofactor for methyl group transfer. These findings contribute to our understanding of the structure-function relationship of CfJHAMT and offer preliminary structural insights that may assist in the development of inhibitors, which could potentially be used to target JH biosynthesis in pest insects. | |||
Molecular insights into juvenile hormone maturation by juvenile hormone acid methyltransferase.,Picard ME, Cusson M, Shi R J Biol Chem. 2026 Apr 17:111474. doi: 10.1016/j.jbc.2026.111474. PMID:42001950<ref>PMID:42001950</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 9xys" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Choristoneura fumiferana]] | |||
[[Category: Large Structures]] | |||
[[Category: Cusson M]] | |||
[[Category: Picard M-E]] | |||
[[Category: Shi R]] | |||
Latest revision as of 07:27, 3 June 2026
Crystal structure of juvenile hormone acid methyltransferase CfJHAMT in complex with SAH and juvenile hormone III acid
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