9wxm: Difference between revisions

Latest revision as of 07:55, 19 November 2025

Cryo-EM structure of the full-length GPR15L bound GPR15-Gi complex

Structural highlights

9wxm is a 6 chain structure with sequence from Homo sapiens and Mus musculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Electron Microscopy, Resolution 3.3Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

GP15L_HUMAN Highly cationic protein that has multiple functions. Acts as a chemotactic factor that mediates lymphocytes recruitment to epithelia through binding and activation of the G-protein coupled receptor GPR15 (PubMed:28900043, PubMed:28936214). May be a tumor suppressor; together with SUSD2 has a growth inhibitory effect on colon cancer cells which includes G1 cell cycle arrest (PubMed:25351403). May regulate keratinocyte proliferation (PubMed:29872130). In addition, through activation of Mas-related G protein-coupled receptors (MRGPRs) contributes to pruritogenesis by activating itch-selective sensory neurons and mast cells degranulation (PubMed:35704588).^[1] ^[2] ^[3] ^[4] ^[5] Has antimicrobial activity against Gram-positive bacteria, including Staphylococcus aureus and Actinomyces spec., and Mycoplasma hominis and lentivirus (PubMed:25585381).^[6]

References

↑ Pan W, Cheng Y, Zhang H, Liu B, Mo X, Li T, Li L, Cheng X, Zhang L, Ji J, Wang P, Han W. CSBF/C10orf99, a novel potential cytokine, inhibits colon cancer cell growth through inducing G1 arrest. Sci Rep. 2014 Oct 29;4:6812. PMID:25351403 doi:10.1038/srep06812
↑ Suply T, Hannedouche S, Carte N, Li J, Grosshans B, Schaefer M, Raad L, Beck V, Vidal S, Hiou-Feige A, Beluch N, Barbieri S, Wirsching J, Lageyre N, Hillger F, Debon C, Dawson J, Smith P, Lannoy V, Detheux M, Bitsch F, Falchetto R, Bouwmeester T, Porter J, Baumgarten B, Mansfield K, Carballido JM, Seuwen K, Bassilana F. A natural ligand for the orphan receptor GPR15 modulates lymphocyte recruitment to epithelia. Sci Signal. 2017 Sep 12;10(496):eaal0180. PMID:28900043 doi:10.1126/scisignal.aal0180
↑ Ocón B, Pan J, Dinh TT, Chen W, Ballet R, Bscheider M, Habtezion A, Tu H, Zabel BA, Butcher EC. A Mucosal and Cutaneous Chemokine Ligand for the Lymphocyte Chemoattractant Receptor GPR15. Front Immunol. 2017 Sep 7;8:1111. PMID:28936214 doi:10.3389/fimmu.2017.01111
↑ Chen C, Wu N, Duan Q, Yang H, Wang X, Yang P, Zhang M, Liu J, Liu Z, Shao Y, Zheng Y. C10orf99 contributes to the development of psoriasis by promoting the proliferation of keratinocytes. Sci Rep. 2018 Jun 5;8(1):8590. PMID:29872130 doi:10.1038/s41598-018-26996-z
↑ Tseng PY, Hoon MA. GPR15L is an epithelial inflammation-derived pruritogen. Sci Adv. 2022 Jun 17;8(24):eabm7342. PMID:35704588 doi:10.1126/sciadv.abm7342
↑ Yang M, Tang M, Ma X, Yang L, He J, Peng X, Guo G, Zhou L, Luo N, Yuan Z, Tong A. AP-57/C10orf99 is a new type of multifunctional antimicrobial peptide. Biochem Biophys Res Commun. 2015 Feb 13;457(3):347-52. PMID:25585381 doi:10.1016/j.bbrc.2014.12.115

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OCA

[1] Pan W, Cheng Y, Zhang H, Liu B, Mo X, Li T, Li L, Cheng X, Zhang L, Ji J, Wang P, Han W. CSBF/C10orf99, a novel potential cytokine, inhibits colon cancer cell growth through inducing G1 arrest. Sci Rep. 2014 Oct 29;4:6812. PMID:25351403 doi:10.1038/srep06812

[2] Suply T, Hannedouche S, Carte N, Li J, Grosshans B, Schaefer M, Raad L, Beck V, Vidal S, Hiou-Feige A, Beluch N, Barbieri S, Wirsching J, Lageyre N, Hillger F, Debon C, Dawson J, Smith P, Lannoy V, Detheux M, Bitsch F, Falchetto R, Bouwmeester T, Porter J, Baumgarten B, Mansfield K, Carballido JM, Seuwen K, Bassilana F. A natural ligand for the orphan receptor GPR15 modulates lymphocyte recruitment to epithelia. Sci Signal. 2017 Sep 12;10(496):eaal0180. PMID:28900043 doi:10.1126/scisignal.aal0180

[3] Ocón B, Pan J, Dinh TT, Chen W, Ballet R, Bscheider M, Habtezion A, Tu H, Zabel BA, Butcher EC. A Mucosal and Cutaneous Chemokine Ligand for the Lymphocyte Chemoattractant Receptor GPR15. Front Immunol. 2017 Sep 7;8:1111. PMID:28936214 doi:10.3389/fimmu.2017.01111

[4] Chen C, Wu N, Duan Q, Yang H, Wang X, Yang P, Zhang M, Liu J, Liu Z, Shao Y, Zheng Y. C10orf99 contributes to the development of psoriasis by promoting the proliferation of keratinocytes. Sci Rep. 2018 Jun 5;8(1):8590. PMID:29872130 doi:10.1038/s41598-018-26996-z

[5] Tseng PY, Hoon MA. GPR15L is an epithelial inflammation-derived pruritogen. Sci Adv. 2022 Jun 17;8(24):eabm7342. PMID:35704588 doi:10.1126/sciadv.abm7342

[6] Yang M, Tang M, Ma X, Yang L, He J, Peng X, Guo G, Zhou L, Luo N, Yuan Z, Tong A. AP-57/C10orf99 is a new type of multifunctional antimicrobial peptide. Biochem Biophys Res Commun. 2015 Feb 13;457(3):347-52. PMID:25585381 doi:10.1016/j.bbrc.2014.12.115

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[6]

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 9wxm is ON HOLD  until Paper Publication
+==Cryo-EM structure of the full-length GPR15L bound GPR15-Gi complex==
+<StructureSection load='9wxm' size='340' side='right'caption='[[9wxm]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
-Authors:
+== Structural highlights ==
+<table><tr><td colspan='2'>[[9wxm]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9WXM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9WXM FirstGlance]. <br>
-Description:
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.3&#8491;</td></tr>
-[[Category: Unreleased Structures]]
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9wxm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9wxm OCA], [https://pdbe.org/9wxm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9wxm RCSB], [https://www.ebi.ac.uk/pdbsum/9wxm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9wxm ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/GP15L_HUMAN GP15L_HUMAN] Highly cationic protein that has multiple functions. Acts as a chemotactic factor that mediates lymphocytes recruitment to epithelia through binding and activation of the G-protein coupled receptor GPR15 (PubMed:28900043, PubMed:28936214). May be a tumor suppressor; together with SUSD2 has a growth inhibitory effect on colon cancer cells which includes G1 cell cycle arrest (PubMed:25351403). May regulate keratinocyte proliferation (PubMed:29872130). In addition, through activation of Mas-related G protein-coupled receptors (MRGPRs) contributes to pruritogenesis by activating itch-selective sensory neurons and mast cells degranulation (PubMed:35704588).<ref>PMID:25351403</ref> <ref>PMID:28900043</ref> <ref>PMID:28936214</ref> <ref>PMID:29872130</ref> <ref>PMID:35704588</ref>   Has antimicrobial activity against Gram-positive bacteria, including Staphylococcus aureus and Actinomyces spec., and Mycoplasma hominis and lentivirus (PubMed:25585381).<ref>PMID:25585381</ref>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Homo sapiens]]
+[[Category: Large Structures]]
+[[Category: Mus musculus]]
+[[Category: Han S]]
+[[Category: Wu B]]
+[[Category: Zhao Q]]

9wxm: Difference between revisions

Latest revision as of 07:55, 19 November 2025

Cryo-EM structure of the full-length GPR15L bound GPR15-Gi complex

Structural highlights

Function

References

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