9x2r: Difference between revisions

Latest revision as of 04:59, 24 June 2026

Crystal structure of MtNSP1-MtNSP2 heterodimer

Structural highlights

9x2r is a 2 chain structure with sequence from Medicago truncatula. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.41Å
Ligands:	EDO, PEG, ZN
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

NSP1_MEDTR Transcriptional regulator essential for Nod-factor-induced gene expression (PubMed:15961669). Acts downstream of calcium spiking (PubMed:15961669). May be a target of DMI3, a calcium/calmodulin-dependent protein kinase (CCaMK) (PubMed:15961669). Is essential for Nod factor-elicited expression of ERN1 (PubMed:23077241). Transcription factor involved in the control of strigolactone biosynthesis in roots through the activation of the beta-carotene isomerase D27, which participates in a pathway leading to biosynthesis of strigolactones (PubMed:22039214, PubMed:26503135).^[1] ^[2] ^[3] ^[4]

References

↑ Smit P, Raedts J, Portyanko V, Debellé F, Gough C, Bisseling T, Geurts R. NSP1 of the GRAS protein family is essential for rhizobial Nod factor-induced transcription. Science. 2005 Jun 17;308(5729):1789-91. PMID:15961669 doi:10.1126/science.1111025
↑ Liu W, Kohlen W, Lillo A, Op den Camp R, Ivanov S, Hartog M, Limpens E, Jamil M, Smaczniak C, Kaufmann K, Yang WC, Hooiveld GJ, Charnikhova T, Bouwmeester HJ, Bisseling T, Geurts R. Strigolactone biosynthesis in Medicago truncatula and rice requires the symbiotic GRAS-type transcription factors NSP1 and NSP2. Plant Cell. 2011 Oct;23(10):3853-65. PMID:22039214 doi:10.1105/tpc.111.089771
↑ Cerri MR, Frances L, Laloum T, Auriac MC, Niebel A, Oldroyd GE, Barker DG, Fournier J, de Carvalho-Niebel F. Medicago truncatula ERN transcription factors: regulatory interplay with NSP1/NSP2 GRAS factors and expression dynamics throughout rhizobial infection. Plant Physiol. 2012 Dec;160(4):2155-72. PMID:23077241 doi:10.1104/pp.112.203190
↑ van Zeijl A, Liu W, Xiao TT, Kohlen W, Yang WC, Bisseling T, Geurts R. The strigolactone biosynthesis gene DWARF27 is co-opted in rhizobium symbiosis. BMC Plant Biol. 2015 Oct 26;15:260. doi: 10.1186/s12870-015-0651-x. PMID:26503135 doi:https://dx.doi.org/10.1186/s12870-015-0651-x

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OCA

[1] Smit P, Raedts J, Portyanko V, Debellé F, Gough C, Bisseling T, Geurts R. NSP1 of the GRAS protein family is essential for rhizobial Nod factor-induced transcription. Science. 2005 Jun 17;308(5729):1789-91. PMID:15961669 doi:10.1126/science.1111025

[2] Liu W, Kohlen W, Lillo A, Op den Camp R, Ivanov S, Hartog M, Limpens E, Jamil M, Smaczniak C, Kaufmann K, Yang WC, Hooiveld GJ, Charnikhova T, Bouwmeester HJ, Bisseling T, Geurts R. Strigolactone biosynthesis in Medicago truncatula and rice requires the symbiotic GRAS-type transcription factors NSP1 and NSP2. Plant Cell. 2011 Oct;23(10):3853-65. PMID:22039214 doi:10.1105/tpc.111.089771

[3] Cerri MR, Frances L, Laloum T, Auriac MC, Niebel A, Oldroyd GE, Barker DG, Fournier J, de Carvalho-Niebel F. Medicago truncatula ERN transcription factors: regulatory interplay with NSP1/NSP2 GRAS factors and expression dynamics throughout rhizobial infection. Plant Physiol. 2012 Dec;160(4):2155-72. PMID:23077241 doi:10.1104/pp.112.203190

[4] van Zeijl A, Liu W, Xiao TT, Kohlen W, Yang WC, Bisseling T, Geurts R. The strigolactone biosynthesis gene DWARF27 is co-opted in rhizobium symbiosis. BMC Plant Biol. 2015 Oct 26;15:260. doi: 10.1186/s12870-015-0651-x. PMID:26503135 doi:https://dx.doi.org/10.1186/s12870-015-0651-x

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@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 9x2r is ON HOLD
+==Crystal structure of MtNSP1-MtNSP2 heterodimer==
+<StructureSection load='9x2r' size='340' side='right'caption='[[9x2r]], [[Resolution|resolution]] 2.41&Aring;' scene=''>
-Authors: Wan, L.H., Hu, Y.X.
+== Structural highlights ==
+<table><tr><td colspan='2'>[[9x2r]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Medicago_truncatula Medicago truncatula]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9X2R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9X2R FirstGlance]. <br>
-Description: Crystal structure of a GRAS protein heterodimer complex
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.41&#8491;</td></tr>
-[[Category: Unreleased Structures]]
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-[[Category: Hu, Y.X]]
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9x2r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9x2r OCA], [https://pdbe.org/9x2r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9x2r RCSB], [https://www.ebi.ac.uk/pdbsum/9x2r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9x2r ProSAT]</span></td></tr>
-[[Category: Wan, L.H]]
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/NSP1_MEDTR NSP1_MEDTR] Transcriptional regulator essential for Nod-factor-induced gene expression (PubMed:15961669). Acts downstream of calcium spiking (PubMed:15961669). May be a target of DMI3, a calcium/calmodulin-dependent protein kinase (CCaMK) (PubMed:15961669). Is essential for Nod factor-elicited expression of ERN1 (PubMed:23077241). Transcription factor involved in the control of strigolactone biosynthesis in roots through the activation of the beta-carotene isomerase D27, which participates in a pathway leading to biosynthesis of strigolactones (PubMed:22039214, PubMed:26503135).<ref>PMID:15961669</ref> <ref>PMID:22039214</ref> <ref>PMID:23077241</ref> <ref>PMID:26503135</ref>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Medicago truncatula]]
+[[Category: Hu YX]]
+[[Category: Wan LH]]

9x2r: Difference between revisions

Latest revision as of 04:59, 24 June 2026

Crystal structure of MtNSP1-MtNSP2 heterodimer

Structural highlights

Function

References

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