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| {{STRUCTURE_1ws7| PDB=1ws7 | SCENE= }} | | {{STRUCTURE_1ws7| PDB=1ws7 | SCENE= }} |
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| '''Crystal Structure of Mavicyanin from Cucurbita pepo medullosa (Zucchini)'''
| | ===Crystal Structure of Mavicyanin from Cucurbita pepo medullosa (Zucchini)=== |
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| ==Overview==
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| Mavicyanin, a glycosylated protein isolated from Cucurbita pepo medullosa (zucchini), is a member of the phytocyanin subfamily containing one polypeptide chain of 109 amino residues and an unusual type-I Cu site in which the copper ligands are His45, Cys86, His91, and Gln96. The crystal structures of oxidized and reduced mavicyanin were determined at 1.6 and 1.9 A resolution, respectively. Mavicyanin has a core structure of seven polypeptide beta-strands arranged as a beta-sandwich organized into two beta-sheets, and the structure considerably resembles that of stellacyanin from cucumber (CST) or cucumber basic protein (CBP). A flexible region was not observed on superimpositioning of the oxidized and reduced mavicyanin structures. However, the Cu(II)-epsilon-O-Gln96 bond length was extended by 0.47 A, and the Thr15 residue was rotated by 60.0 degrees and O-gamma1-Thr15 moved from a distance of 4.78 to 2.58 A from the ligand Gln96 forming a new hydrogen bond between O-gamma1-Thr15 and epsilon-O-Gln96 upon reduction. The reorganization of copper coordination geometry of mavicyanin upon reduction arouses reduction potential decreased above pH 8 [Battistuzzi et al. (2001) J. Inorg. Biochem. 83, 223-227]. The rotation of Thr15 and the hydrogen bonding with the ligand Gln96 may constitute structural evidence of the decrease in the reduction potential at high pH.
| | The line below this paragraph, {{ABSTRACT_PUBMED_15858169}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 15858169 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_15858169}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Phytocyanin]] | | [[Category: Phytocyanin]] |
| [[Category: Reduced form]] | | [[Category: Reduced form]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 14:04:27 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 22:14:49 2008'' |
