1zzi: Difference between revisions

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[[Image:1zzi.gif|left|200px]]<br />

[[Image:1zzi.gif|left|200px]]<br /><applet load="1zzi" size="350" color="white" frame="true" align="right" spinBox="true"

<applet load="1zzi" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

caption="1zzi, resolution 1.80Å" />

'''Crystal Structure Analysis of the third KH domain of hnRNP K in complex with ssDNA'''<br />

==Overview==

The heterogeneous nuclear ribonucleoprotein (hnRNP) K is implicated in, multiple functions in the regulation of gene expression and acts as a hub, at the intersection of signaling pathways and processes involving nucleic, acids. Central to its function is its ability to bind both ssDNA and ssRNA, via its KH (hnRNP K homology) domains. We determined crystal structures of, hnRNP K KH3 domain complexed with 15-mer and 6-mer (CTC(4)) ssDNAs at 2.4, and 1.8 A resolution, respectively, and show that the KH3 domain binds, specifically to both TCCC and CCCC sequences. In parallel, we used NMR to, compare the binding affinity and mode of interaction of the KH3 domain, with several ssRNA ligands and CTC(4) ssDNA. Based on a structure, alignment of the KH3-CTC(4) complex with known structures of other KH, domains in complex with ssRNA, we discuss recognition of tetranucleotide, sequences by KH domains.

The heterogeneous nuclear ribonucleoprotein (hnRNP) K is implicated in multiple functions in the regulation of gene expression and acts as a hub at the intersection of signaling pathways and processes involving nucleic acids. Central to its function is its ability to bind both ssDNA and ssRNA via its KH (hnRNP K homology) domains. We determined crystal structures of hnRNP K KH3 domain complexed with 15-mer and 6-mer (CTC(4)) ssDNAs at 2.4 and 1.8 A resolution, respectively, and show that the KH3 domain binds specifically to both TCCC and CCCC sequences. In parallel, we used NMR to compare the binding affinity and mode of interaction of the KH3 domain with several ssRNA ligands and CTC(4) ssDNA. Based on a structure alignment of the KH3-CTC(4) complex with known structures of other KH domains in complex with ssRNA, we discuss recognition of tetranucleotide sequences by KH domains.

==About this Structure==

1ZZI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1ZZI OCA].

1ZZI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZZI OCA].

==Reference==

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Line 13:

[[Category: Homo sapiens]]

[[Category: Single protein]]

[[Category: Backe, P.H.]]

[[Category: Backe, P H.]]

[[Category: Cusack, S.]]

[[Category: Messias, A.C.]]

[[Category: Messias, A C.]]

[[Category: Ravelli, R.B.]]

[[Category: Ravelli, R B.]]

[[Category: Sattler, M.]]

[[Category: protein-ssdna complex]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Mon Nov 12~~ 20:~~43:22 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:20:40 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1zzi.gif|left|200px]]<br />
+[[Image:1zzi.gif|left|200px]]<br /><applet load="1zzi" size="350" color="white" frame="true" align="right" spinBox="true"
-<applet load="1zzi" size="450" color="white" frame="true" align="right" spinBox="true"
 caption="1zzi, resolution 1.80&Aring;" />
 '''Crystal Structure Analysis of the third KH domain of hnRNP K in complex with ssDNA'''<br />
 ==Overview==
-The heterogeneous nuclear ribonucleoprotein (hnRNP) K is implicated in, multiple functions in the regulation of gene expression and acts as a hub, at the intersection of signaling pathways and processes involving nucleic, acids. Central to its function is its ability to bind both ssDNA and ssRNA, via its KH (hnRNP K homology) domains. We determined crystal structures of, hnRNP K KH3 domain complexed with 15-mer and 6-mer (CTC(4)) ssDNAs at 2.4, and 1.8 A resolution, respectively, and show that the KH3 domain binds, specifically to both TCCC and CCCC sequences. In parallel, we used NMR to, compare the binding affinity and mode of interaction of the KH3 domain, with several ssRNA ligands and CTC(4) ssDNA. Based on a structure, alignment of the KH3-CTC(4) complex with known structures of other KH, domains in complex with ssRNA, we discuss recognition of tetranucleotide, sequences by KH domains.
+The heterogeneous nuclear ribonucleoprotein (hnRNP) K is implicated in multiple functions in the regulation of gene expression and acts as a hub at the intersection of signaling pathways and processes involving nucleic acids. Central to its function is its ability to bind both ssDNA and ssRNA via its KH (hnRNP K homology) domains. We determined crystal structures of hnRNP K KH3 domain complexed with 15-mer and 6-mer (CTC(4)) ssDNAs at 2.4 and 1.8 A resolution, respectively, and show that the KH3 domain binds specifically to both TCCC and CCCC sequences. In parallel, we used NMR to compare the binding affinity and mode of interaction of the KH3 domain with several ssRNA ligands and CTC(4) ssDNA. Based on a structure alignment of the KH3-CTC(4) complex with known structures of other KH domains in complex with ssRNA, we discuss recognition of tetranucleotide sequences by KH domains.
 ==About this Structure==
-ZZI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1ZZI OCA].
+ZZI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZZI OCA].
 ==Reference==
@@ Line 14: / Line 13: @@
 [[Category: Homo sapiens]]
 [[Category: Single protein]]
-[[Category: Backe, P.H.]]
+[[Category: Backe, P H.]]
 [[Category: Cusack, S.]]
-[[Category: Messias, A.C.]]
+[[Category: Messias, A C.]]
-[[Category: Ravelli, R.B.]]
+[[Category: Ravelli, R B.]]
 [[Category: Sattler, M.]]
 [[Category: protein-ssdna complex]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 20:43:22 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:20:40 2008''