9x67: Difference between revisions

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'''Unreleased structure'''


The entry 9x67 is ON HOLD  until Paper Publication
==Cryo-EM structure of the type I pilus from Escherichia Coli and the surrounding water network==
<StructureSection load='9x67' size='340' side='right'caption='[[9x67]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9x67]] is a 15 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9X67 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9X67 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 1.85&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9x67 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9x67 OCA], [https://pdbe.org/9x67 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9x67 RCSB], [https://www.ebi.ac.uk/pdbsum/9x67 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9x67 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/FIMA1_ECOLI FIMA1_ECOLI] Fimbriae (also called pili), polar filaments radiating from the surface of the bacterium to a length of 0.5-1.5 micrometers and numbering 100-300 per cell, enable bacteria to colonize the epithelium of specific host organs.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Type 1 pili are protein filamentous surface structures of Gram-negative bacteria that mediate adhesion to host and play a crucial role in infection. Here, we report the cryogenic electron microscopy structure of the type 1 pilus from uropathogenic E. coli K-12 comprising 15 subunits of the major protein pilin FimA. The final local resolution of electron microscopy reconstruction was estimated to reach 1.85 A, which is higher than that of the previously published structure. This improvement in the resolution enabled us to refine side-chain conformations to reliably determine the distances between the side-chain residues participating in the intersubunit interactions and determine a network of water molecules surrounding the pilus rod. The analysis revealed that water contributes to intersubunit stabilization both through discrete bridging interactions and through extended hydrogen-bonded clusters, thereby supporting both the rigidity and flexibility of the filament. Comparison with a homologous high-resolution pilus model from enterotoxigenic E. coli showed that the vast majority of 'conserved' water molecules, that is, those that are present at equivalent positions in different subunits of our model occupy also equivalent positions across the two structures, underscoring their functional relevance. At the same time, sequence-specific differences in hydration patterns were observed. These findings highlight the structural and functional importance of water in pilus architecture and provide a more detailed molecular framework for understanding bacterial adhesion.


Authors: Petrova, T.E., Glukhov, A.S., Stetsenko, A., Guskov, A., Gabdulkhakov, A.G.
Determination of the water network surrounding the type I pilus from Escherichia coli by cryo-electron microscopy.,Petrova TE, Glukhov AS, Stetsenko A, Guskov A, Gabdulkhakov AG FEBS J. 2026 Feb 24. doi: 10.1111/febs.70473. PMID:41733276<ref>PMID:41733276</ref>


Description: Cryo-EM structure of the type I pilus from Escherichia Coli and the surrounding water network
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
[[Category: Glukhov, A.S]]
<div class="pdbe-citations 9x67" style="background-color:#fffaf0;"></div>
[[Category: Petrova, T.E]]
== References ==
[[Category: Guskov, A]]
<references/>
[[Category: Gabdulkhakov, A.G]]
__TOC__
[[Category: Stetsenko, A]]
</StructureSection>
[[Category: Escherichia coli K-12]]
[[Category: Large Structures]]
[[Category: Gabdulkhakov AG]]
[[Category: Glukhov AS]]
[[Category: Guskov A]]
[[Category: Petrova TE]]
[[Category: Stetsenko A]]
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