9wy8: Difference between revisions
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==Cryo-EM structure of the hexameric DRT6== | |||
<StructureSection load='9wy8' size='340' side='right'caption='[[9wy8]], [[Resolution|resolution]] 2.69Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[9wy8]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9WY8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9WY8 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.69Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9wy8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9wy8 OCA], [https://pdbe.org/9wy8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9wy8 RCSB], [https://www.ebi.ac.uk/pdbsum/9wy8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9wy8 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/MALE_ECOLI MALE_ECOLI] Involved in the high-affinity maltose membrane transport system MalEFGK. Initial receptor for the active transport of and chemotaxis toward maltooligosaccharides. | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Defense-associated reverse transcriptases (DRTs) were recently identified with anti-phage function. Among them, DRT6 represents a single-gene-encoded anti-phage system that confers resistance to multiple DNA bacteriophages; however, its structural basis and mechanism of action remain unknown. Here, we determined a high-resolution cryo-EM structure of DRT6, revealing that it assembles into a hexamer composed of two trimers arranged in a back-to-back configuration. Structure-guided mutagenesis demonstrated that the integrity of this hexameric interface is critical for both the stability and anti-phage activity of DRT6. Comparative structural analysis showed that DRT6 shares notable similarity with the anti-phage protein AbiK. Intriguingly, the thumb subdomain of its reverse transcriptase domain is replaced by an alpha-helical repeat (alphaRep) domain, forming a positively charged channel that likely mediates nucleic acid binding. Nevertheless, DRT6 differs from AbiK in the position of its priming amino acid, suggesting distinct functional mechanisms. Together, this work reports the first atomic structure of DRT6, elucidates the molecular basis of its functional oligomerization, and provides insights into the anti-phage mechanism. | |||
Cryo-EM structure of the bacterial anti-phage defense system DRT6.,Wang Y, Wu H, Li J, Tian Z, Deng Z Biochem Biophys Res Commun. 2025 Nov 28;791:152955. doi: , 10.1016/j.bbrc.2025.152955. Epub 2025 Nov 9. PMID:41223699<ref>PMID:41223699</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 9wy8" style="background-color:#fffaf0;"></div> | ||
[[Category: Deng | == References == | ||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Escherichia coli]] | |||
[[Category: Escherichia coli K-12]] | |||
[[Category: Large Structures]] | |||
[[Category: Deng Z]] | |||
[[Category: Wang Y]] | |||
Latest revision as of 13:29, 10 February 2026
Cryo-EM structure of the hexameric DRT6
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