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| [[Image:1wyw.gif|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_1wyw| PDB=1wyw | SCENE= }} | | {{STRUCTURE_1wyw| PDB=1wyw | SCENE= }} |
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| '''Crystal Structure of SUMO1-conjugated thymine DNA glycosylase'''
| | ===Crystal Structure of SUMO1-conjugated thymine DNA glycosylase=== |
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| ==Overview==
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| Members of the small ubiquitin-like modifier (SUMO) family can be covalently attached to the lysine residue of a target protein through an enzymatic pathway similar to that used in ubiquitin conjugation, and are involved in various cellular events that do not rely on degradative signalling via the proteasome or lysosome. However, little is known about the molecular mechanisms of SUMO-modification-induced protein functional transfer. During DNA mismatch repair, SUMO conjugation of the uracil/thymine DNA glycosylase TDG promotes the release of TDG from the abasic (AP) site created after base excision, and coordinates its transfer to AP endonuclease 1, which catalyses the next step in the repair pathway. Here we report the crystal structure of the central region of human TDG conjugated to SUMO-1 at 2.1 A resolution. The structure reveals a helix protruding from the protein surface, which presumably interferes with the product DNA and thus promotes the dissociation of TDG from the DNA molecule. This helix is formed by covalent and non-covalent contacts between TDG and SUMO-1. The non-covalent contacts are also essential for release from the product DNA, as verified by mutagenesis.
| | The line below this paragraph, {{ABSTRACT_PUBMED_15959518}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 15959518 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_15959518}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Uchimura, Y.]] | | [[Category: Uchimura, Y.]] |
| [[Category: Hydrolase]] | | [[Category: Hydrolase]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 14:19:39 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 00:28:41 2008'' |
